A Micro-electroporation/electrophoresis-based Vaccine Screening System Reveals the Impact of Vaccination Orders on Cross-protective Immunity

Overview

Journal iScience

Publisher Cell Press

Date 2023 Oct 20

PMID 37860767

Authors

Yongyong Li

Jingshan Mo

Jing Liu

Ying Liang

Caiguanxi Deng

Zhangping Huang

Juan Jiang

Ming Liu

Xinmin Liu

Liru Shang

Xiafeng Wang

Xi Xie

Ji Wang

Affiliations

Soon will be listed here.

Abstract

The constant emergence of mutated pathogens poses great challenges to the existing vaccine system. A screening system is needed to screen for antigen designs and vaccination strategies capable of inducing cross-protective immunity. Herein, we report a screening system based on DNA vaccines and a micro-electroporation/electrophoresis system (MEES), which greatly improved the efficacy of DNA vaccines, elevating humoral and cellular immune responses by over 400- and 35-fold respectively. Eighteen vaccination strategies were screened simultaneously by sequential immunization with vaccines derived from wildtype (WT) SARS-CoV-2, Delta, or Omicron BA.1 variant. Sequential vaccination of BA.1-WT-Delta vaccines with MEES induced potent neutralizing antibodies against all three viral strains and BA.5 variant, demonstrating that cross-protective immunity against future mutants can be successfully induced by existing strain-derived vaccines when a proper combination and order of sequential vaccination are used. Our screening system could be used for fast-seeking vaccination strategies for emerging pathogens in the future.

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