Green Leaf Volatile Sensory Calcium Transduction in Arabidopsis

Overview

Journal Nat Commun

Specialty Biology

Date 2023 Oct 17

PMID 37848440

Authors

Yuri Aratani

Takuya Uemura

Takuma Hagihara

Kenji Matsui

Masatsugu Toyota

Affiliations

Soon will be listed here.

Abstract

Plants perceive volatile organic compounds (VOCs) released by mechanically- or herbivore-damaged neighboring plants and induce various defense responses. Such interplant communication protects plants from environmental threats. However, the spatiotemporal dynamics of VOC sensory transduction in plants remain largely unknown. Using a wide-field real-time imaging method, we visualize an increase in cytosolic Ca concentration ([Ca]) in Arabidopsis leaves following exposure to VOCs emitted by injured plants. We identify two green leaf volatiles (GLVs), (Z)-3-hexenal (Z-3-HAL) and (E)-2-hexenal (E-2-HAL), which increase [Ca] in Arabidopsis. These volatiles trigger the expression of biotic and abiotic stress-responsive genes in a Ca-dependent manner. Tissue-specific high-resolution Ca imaging and stomatal mutant analysis reveal that [Ca] increases instantly in guard cells and subsequently in mesophyll cells upon Z-3-HAL exposure. These results suggest that GLVs in the atmosphere are rapidly taken up by the inner tissues via stomata, leading to [Ca] increases and subsequent defense responses in Arabidopsis leaves.

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