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OffRisk: a Docker Image for Annotating CRISPR Off-target Sites in the Human Genome

Overview
Journal Bioinform Adv
Publisher Oxford University Press
Specialty Biology
Date 2023 Oct 16
PMID 37840905
Authors
Gil-Ad Barkai
Tal Malul
Yossi Eliaz
Eran Eyal
Isana Veksler-Lublinsky
Affiliations
Soon will be listed here.
Abstract

Summary: The CRISPR-Cas9 system has been adapted to achieve targeted genome editing as well as transcriptional control by customizing 20-nt guide RNA (gRNA) molecules for desired regions in the target genome. Designing gRNAs must consider nonspecific and unintended binding, known as off-targets, since these may have potentially harmful effects. To assist in gRNA design, we have developed OffRisk. This Docker-based tool annotates off-target sites in the human genome and assigns them a potential risk label by incorporating functional and regulatory information at different molecular levels.

Availability And Implementation: OffRisk is available at https://github.com/IsanaVekslerLublinsky/OffRisk and https://github.com/IsanaVekslerLublinsky/OffRisk-ui (including code, user guide, docker installation guide, and running examples).All processed datasets are available at https://zenodo.org/record/8289271.

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