Flavonoid Biosynthesis Pathway May Indirectly Affect Outcrossing Rate of Cytoplasmic Male-Sterile Lines of Soybean

Overview

Journal Plants (Basel)

Date 2023 Oct 14

PMID 37836201

Authors

Chunjing Lin

Yuetong Duan

Rong Li

Pengnian Wang

Yanyan Sun

Xiaoyang Ding

Jingyong Zhang

Hao Yan

Wei Zhang

Bao Peng

Limei Zhao

Chunbao Zhang

Affiliations

Soon will be listed here.

Abstract

(1) Background: Cytoplasmic male sterility (CMS) is important for exploiting heterosis. Soybean ( L.) has a low outcrossing rate that is detrimental for breeding sterile lines and producing hybrid seeds. Therefore, the molecular mechanism controlling the outcrossing rate should be elucidated to increase the outcrossing rate of soybean CMS lines; (2) Methods: The male-sterile soybean lines JLCMS313A (with a high outcrossing rate; HL) and JLCMS226A (with a low outcrossing rate; LL) were used for a combined analysis of the transcriptome (RNA-seq) and the targeted phenol metabolome; (3) Results: The comparison between HL and LL detected 5946 differentially expressed genes (DEGs) and 81 phenolic metabolites. The analysis of the DEGs and differentially abundant phenolic metabolites identified only one common KEGG pathway related to flavonoid biosynthesis. The qRT-PCR expression for eight DEGs was almost consistent with the transcriptome data. The comparison of the cloned coding sequence (CDS) regions of the , , , and genes between HL and LL revealed seven single nucleotide polymorphisms (SNPs) only in the CDS. Moreover, five significant differentially abundant phenolic metabolites between HL and LL were associated with flavonoid metabolic pathways. Finally, on the basis of the SNPs in the CDS, one derived cleaved amplified polymorphic sequence (dCAPS) marker was developed to distinguish between HL and LL soybean lines; (4) Conclusions: The flavonoid biosynthesis pathway may indirectly affect the outcrossing rate of CMS sterile lines in soybean.

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