Baicalin-aluminum Alleviates Necrotic Enteritis in Broiler Chickens by Inhibiting Virulence Factors Expression of

Overview

Journal Front Cell Infect Microbiol

Specialties Cell Biology
Infectious Diseases
Microbiology

Date 2023 Oct 11

PMID 37818042

Authors

Jin Liu

Shuangqi Wu

Honghao Zhao

Chun Ye

Shulin Fu

Yu Liu

Ting Liu

Yinsheng Qiu

Affiliations

Soon will be listed here.

Abstract

type A is the main cause of necrotic enteritis (NE) in chickens. Since the use of antibiotics in feed is withdrawn, it is imperative to find out suitable alternatives to control NE. Baicalin-aluminum complex is synthesized from baicalin, a flavonoid isolated from Georgi. The present study investigated the effects of baicalin-aluminum on the virulence-associated traits and virulence genes expression of CVCC2030, it also evaluated the therapeutic effect on NE. The results showed that baicalin-aluminum inhibited bacterial hemolytic activity, diminished biofilm formation, attenuated cytotoxicity to Caco-2 cells, downregulated the expression of genes encoding for clostridial toxins and extracellular enzymes such as alpha toxin (CPA), perfringolysin O (PFO), collagenase (ColA), and sialidases (NanI, NanJ). Additionally, baicalin-aluminum was found to negatively regulate the expression of genes involved in quorum sensing (QS) communication, including genes of Agr QS system (, ) and genes of VirS/R two-component regulatory system (, ). experiments, baicalin-aluminum lightened the intestinal lesions and histological damage, it inhibited pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) expression in the jejunal and ileal tissues. Besides, baicalin-aluminum alleviated the upregulation of and and raised the relative abundance of in the ileal digesta. This study suggests that baicalin-aluminum may be a potential candidate against infection by inhibiting the virulence-associated traits and virulence genes expression.

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