The BZIP-type Transcription Factors NapA and RsmA Modulate the Volumetric Ratio and the Relative Superoxide Ratio of Mitochondria in Aspergillus Nidulans

Overview

Journal Biol Futur

Specialty Biology

Date 2023 Oct 9

PMID 37814124

Authors

Bernadett Bakany

Reka Antal

Peter Szentesi

Tamas Emri

Eva Leiter

Laszlo Csernoch

Nancy P Keller

Istvan Pocsi

Beatrix Dienes

Affiliations

Soon will be listed here.

Abstract

Basic leucine zipper (bZIP) transcription factors are crucial components of differentiation, cellular homeostasis and the environmental stress defense of eukaryotes. In this work, we further studied the consequence of gene deletion and overexpression of two bZIP transcription factors, NapA and RsmA, on superoxide production, mitochondrial morphology and hyphal diameter of Aspergillus nidulans. We have found that reactive oxygen species production was influenced by both gene deletion and overexpression of napA under tert-butylhydroperoxide (tBOOH) elicited oxidative stress. Furthermore, gene expression of napA negatively correlated with mitochondrial volumetric ratio as well as sterigmatocystin production of A. nidulans. High rsmA expression was accompanied with elevated relative superoxide ratio in the second hyphal compartment. A negative correlation between the expression of rsmA and catalase enzyme activity or mitochondrial volumetric ratio was also confirmed by statistical analysis. Hyphal diameter was independent on either rsmA and napA expression as well as 0.2 mM tBOOH treatment.

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