The Effects of Ninjinyoeito on Impaired Spatial Memory and Prefrontal Cortical Synaptic Plasticity Through -Amino-3-hydroxy-5-4-isoxazole Propionic Acid Receptor Subunit in a Rat Model with Cerebral Ischemia and -Amyloid Injection

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2023 Oct 9

PMID 37808130

Authors

Masaki Nagao

Akinobu Hatae

Kazuma Mine

Soichiro Tsutsumi

Hiroya Omori

Marika Hirata

Maaya Arimatsu

Chise Taniguchi

Takuya Watanabe

Kaori Kubota

Shutaro Katsurabayashi

Katsunori Iwasaki

Affiliations

Soon will be listed here.

Abstract

Ninjinyoeito (NYT), a traditional Japanese medicine, is effective for improving physical strength and treating fatigue and anorexia. Recently, a clinical report revealed that NYT ameliorates cognitive dysfunction in Alzheimer's disease (AD) patients, although the mechanisms remain unclear. AD is a neurodegenerative disorder accompanied by a progressive deficit in memory. Current therapeutic agents are largely ineffective in treating cognitive dysfunction in AD patients. In this study, we investigated the effects of NYT on spatial memory impairment in a rat model of dementia. Rats were prepared with transient cerebral ischemia and intraventricular injection of -amyloid for 7 days (CI + A). NYT was orally administered for 7 days after cerebral ischemia. We evaluated spatial memory using the Morris water maze and investigated the expression of -amino-3-hydroxy-5-4-isoxazole propionic acid receptor subunits, the phosphorylation level of glutamate receptor A (GluA)1 at serine sites S831 and S845, and the Ca/calmodulin-dependent protein kinase II (CaMKII) in the hippocampus and prefrontal cortex of CI + A rats. In the CI + A rats, NYT treatment shortened the extended time to reach the platform. However, NYT did not restore the decrease in the hippocampal GluA1, GluA2, or CaMKII expression but increased prefrontal cortical phosphorylation levels of S845-GluA1 and CaMKII. Therefore, NYT may alleviate spatial memory impairment by promoting glutamatergic transmission involved in the phosphorylation of S845-GluA1 and CaMKII in the prefrontal cortex of CI + A rats. Our results suggest that NYT is a valuable treatment for AD patients.

Citing Articles

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