Hyperthermophile Diversity Microbes in the Calientes Geothermal Field, Tacna, Peru

Overview

Journal Braz J Microbiol

Specialty Microbiology

Date 2023 Oct 6

PMID 37801222

Authors

Silvia Valdez

Fabian Veliz de la Vega

Omar Pairazaman

Roberto Castellanos

Mario Esparza

Affiliations

Soon will be listed here.

Abstract

Hyperthermophile microorganisms have been discovered worldwide, and several studies regarding biodiversity and the potential biotechnological applications have been reported. In this work, we describe for the first time the diversity of hyperthermophile communities in the Calientes Geothermal Field (CGF) located 4400 m above sea level in Tacna Region, Perú. Three hot springs were monitored and showed a temperature around 84 to 88 °C, for the microbiome analyzed was taken by sampling of sediment and water (pH 7.3-7.6). The hyperthermophile diversity was determined by PCR, DGGE, and DNA sequencing. The sediments analyzed showed a greater diversity than water samples. Sediments showed a more abundant population of bacteria than archaea, with the presence of at least 9 and 5 phylotypes, respectively. Most interestingly, in some taxa of bacteria (Bacillus) and archaea (Haloarcula and Halalkalicoccus), any of operational taxonomic units (OTUs) have not been observed before in hyperthermophile environments. Our results provide insight in the hyperthermophile diversity and reveal the possibility to develop new biotechnological applications based on the kind of environments.

References

White 3rd R, Soles S, Gavelis G, Gosselin E, Slater G, Lim D . The Complete Genome and Physiological Analysis of the Eurythermal Firmicute Strain RW2 Isolated From a Freshwater Microbialite, Widely Adaptable to Broad Thermal, pH, and Salinity Ranges. Front Microbiol. 2019; 9:3189. PMC: 6331483. DOI: 10.3389/fmicb.2018.03189. View

Kasana R, Pandey C . Exiguobacterium: an overview of a versatile genus with potential in industry and agriculture. Crit Rev Biotechnol. 2017; 38(1):141-156. DOI: 10.1080/07388551.2017.1312273. View

Colman D, Lindsay M, Harnish A, Bilbrey E, Amenabar M, Selensky M . Seasonal hydrologic and geologic forcing drive hot spring geochemistry and microbial biodiversity. Environ Microbiol. 2021; 23(7):4034-4053. DOI: 10.1111/1462-2920.15617. View

Bull A, Asenjo J, Goodfellow M, Gomez-Silva B . The Atacama Desert: Technical Resources and the Growing Importance of Novel Microbial Diversity. Annu Rev Microbiol. 2016; 70:215-34. DOI: 10.1146/annurev-micro-102215-095236. View

Kang E, Jin H, La J, Sung J, Park S, Kim W . Identification of keratinases from Fervidobacterium islandicum AW-1 using dynamic gene expression profiling. Microb Biotechnol. 2019; 13(2):442-457. PMC: 7017815. DOI: 10.1111/1751-7915.13493. View

Yin Z, Ye L, Jing C . Genome-Resolved Metagenomics and Metatranscriptomics Reveal that Aquificae Dominates Arsenate Reduction in Tengchong Geothermal Springs. Environ Sci Technol. 2022; 56(22):16473-16482. DOI: 10.1021/acs.est.2c05764. View

Hynek B, Rogers K, Antunovich M, Avard G, Alvarado G . Lack of Microbial Diversity in an Extreme Mars Analog Setting: Poás Volcano, Costa Rica. Astrobiology. 2018; 18(7):923-933. PMC: 6067093. DOI: 10.1089/ast.2017.1719. View

Kozubal M, Romine M, Jennings R, Jay Z, Tringe S, Rusch D . Geoarchaeota: a new candidate phylum in the Archaea from high-temperature acidic iron mats in Yellowstone National Park. ISME J. 2012; 7(3):622-34. PMC: 3578567. DOI: 10.1038/ismej.2012.132. View

Podar P, Yang Z, Bjornsdottir S, Podar M . Comparative Analysis of Microbial Diversity Across Temperature Gradients in Hot Springs From Yellowstone and Iceland. Front Microbiol. 2020; 11:1625. PMC: 7372906. DOI: 10.3389/fmicb.2020.01625. View

10.

Mallik S, Kundu S . Molecular interactions within the halophilic, thermophilic, and mesophilic prokaryotic ribosomal complexes: clues to environmental adaptation. J Biomol Struct Dyn. 2014; 33(3):639-56. DOI: 10.1080/07391102.2014.900457. View

11.

Habib N, Khan I, Hussain F, Zhou E, Xiao M, Dong L . Meiothermus luteus sp. nov., a slightly thermophilic bacterium isolated from a hot spring. Int J Syst Evol Microbiol. 2017; 67(8):2910-2914. DOI: 10.1099/ijsem.0.002040. View

12.

Raghunathan G, Marx A . Identification of Thermus aquaticus DNA polymerase variants with increased mismatch discrimination and reverse transcriptase activity from a smart enzyme mutant library. Sci Rep. 2019; 9(1):590. PMC: 6345897. DOI: 10.1038/s41598-018-37233-y. View

13.

Strunecky O, Kopejtka K, Goecke F, Tomasch J, Lukavsky J, Neori A . High diversity of thermophilic cyanobacteria in Rupite hot spring identified by microscopy, cultivation, single-cell PCR and amplicon sequencing. Extremophiles. 2018; 23(1):35-48. DOI: 10.1007/s00792-018-1058-z. View

14.

Kumar S, Stecher G, Li M, Knyaz C, Tamura K . MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018; 35(6):1547-1549. PMC: 5967553. DOI: 10.1093/molbev/msy096. View

15.

Kublanov I, Prokofeva M, Kostrikina N, Kolganova T, Tourova T, Wiegel J . Thermoanaerobacterium aciditolerans sp. nov., a moderate thermoacidophile from a Kamchatka hot spring. Int J Syst Evol Microbiol. 2007; 57(Pt 2):260-264. DOI: 10.1099/ijs.0.64633-0. View

16.

Massello F, Chan C, Chan K, Goh K, Donati E, Urbieta M . Meta-Analysis of Microbial Communities in Hot Springs: Recurrent Taxa and Complex Shaping Factors beyond pH and Temperature. Microorganisms. 2020; 8(6). PMC: 7356817. DOI: 10.3390/microorganisms8060906. View

17.

Liu B, Tang S, Zhang Y, Lu X, Li L, Cheng J . Halalkalicoccus paucihalophilus sp. nov., a halophilic archaeon from Lop Nur region in Xinjiang, northwest of China. Antonie Van Leeuwenhoek. 2013; 103(5):1007-14. DOI: 10.1007/s10482-013-9880-x. View

18.

Keller M, Lipscomb G, Loder A, Schut G, Kelly R, Adams M . A hybrid synthetic pathway for butanol production by a hyperthermophilic microbe. Metab Eng. 2014; 27:101-106. DOI: 10.1016/j.ymben.2014.11.004. View

19.

Zadvornyy O, Boyd E, Posewitz M, Zorin N, Peters J . Biochemical and Structural Characterization of Enolase from Chloroflexus aurantiacus: Evidence for a Thermophilic Origin. Front Bioeng Biotechnol. 2015; 3:74. PMC: 4450660. DOI: 10.3389/fbioe.2015.00074. View

20.

Khelifi N, Ben Romdhane E, Hedi A, Postec A, Fardeau M, Hamdi M . Characterization of Microaerobacter geothermalis gen. nov., sp. nov., a novel microaerophilic, nitrate- and nitrite-reducing thermophilic bacterium isolated from a terrestrial hot spring in Tunisia. Extremophiles. 2010; 14(3):297-304. DOI: 10.1007/s00792-010-0308-5. View