Production, Characterization and Prebiotic Potential of Xylooligosaccharides Produced from Wheat Bran Using KS1 Xylanase

Overview

Journal Indian J Microbiol

Specialty Microbiology

Date 2023 Oct 2

PMID 37781003

Authors

Kajal Kumari

Sushil Nagar

Sakshi Goyal

Sonu Maan

Meena Sindhu

Renu Singh

Vinod Kumar

Affiliations

Soon will be listed here.

Abstract

In the present investigation, xylooligosaccharides were produced from wheat bran and wheat bran extracted xylan through enzymatic hydrolysis using xylanase from novel KS1. Xylooligosaccharides/reducing sugars production from wheat bran was found maximum (374 mg/g) when 4.0% of wheat bran was treated with 375 units (IU/mL) of KS1 xylanase at pH 6.0 and incubated at 50 °C for 24 h of incubation. In case of wheat bran extracted xylan 419 mg/g of xylooligosaccharides were produced when 3% of extracted xylan was incubate for 8 h. Analysis of the enzymatic hydrolysate through high performance liquid chromatography equipped with refractive index detector showed the presence of xylose, xylopentose and xylohexose. The decrease in pH with 1.0% dose of xylooligosacchaides produced from extracted xylan hydrolysis using KS1 xylanase showed more decrease with (6.72 to 5.94) followed by (6.71 to 6.15) and (6.71 to 6.41). In case of increase in optical density both wheat bran and wheat bran extracted xylan generated xylooligosaccharides exhibited similar pattern i.e., > > .

Citing Articles

Xylooligosaccharide Production From Lignocellulosic Biomass and Their Health Benefits as Prebiotics.

Kumari K, Nagar S, Goyal S, Maan S, Chugh V, Kumar V Biochem Res Int. 2024; 2024:6179375.

PMID: 39534514 PMC: 11557181. DOI: 10.1155/2024/6179375.

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