Single-Nucleotide Resolution Mapping of -Methyladenine in Genomic DNA

Overview

Journal ACS Cent Sci

Specialty Chemistry

Date 2023 Oct 2

PMID 37780356

Authors

Cheng-Jie Ma

Gaojie Li

Wen-Xuan Shao

Yi-Hao Min

Ping Wang

Jiang-Hui Ding

Neng-Bin Xie

Min Wang

Feng Tang

Yu-Qi Feng

Weimin Ci

Yinsheng Wang

Bi-Feng Yuan

Affiliations

Soon will be listed here.

Abstract

-Methyladenine (6mA) is a naturally occurring DNA modification in both prokaryotes and eukaryotes. Herein, we developed a deaminase-mediated sequencing (DM-seq) method for genome-wide mapping of 6mA at single-nucleotide resolution. The method capitalizes on the selective deamination of adenine, but not 6mA, in DNA mediated by an evolved adenine deaminase, ABE8e. By employing this method, we achieved genome-wide mapping of 6mA in and in mammalian mitochondrial DNA (mtDNA) at single-nucleotide resolution. We found that the 6mA sites are mainly located in the GATC motif in the genome. We also identified 17 6mA sites in mtDNA of HepG2 cells, where all of the 6mA sites are distributed in the heavy strand of mtDNA. We envision that DM-seq will be a valuable tool for uncovering new functions of 6mA in DNA and for exploring its potential roles in mitochondria-related human diseases.

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