Proteomic Profile of Epidermal Mucus from Reveals Differentially Abundant Proteins After Infection

Overview

Journal Fish Shellfish Immunol Rep

Date 2023 Sep 29

PMID 37771818

Authors

Shandana Ali

Waheed Ullah

Ahmad Faris Seman Kamarulzaman

Maizom Hassan

Muhammad Rauf

Muhammad Nasir Khan Khattak

Farman Ullah Dawar

Affiliations

Soon will be listed here.

Abstract

We report the proteomic profile of Epidermal Mucus (EM) from and identified the differentially abundant proteins (DAPs) against infection through label-free liquid chromatography-mass spectrometry (LC-MS/MS). Using discovery-based proteomics, a total of 2039 proteins were quantified in nontreated group and 1,328 proteins in the treated group, of which 114 were identified as DAPs in both the groups. Of the 114 DAPs, 68 proteins were upregulated and 46 proteins were downregulated in the treated group compared to nontreated group. Functional annotations of these DAPs shows their association with metabolism, cellular process, molecular process, cytoskeletal, stress, and particularly immune system. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and Fisher's exact test between the two groups shows that most of the proteins were immune-related, which were significantly associated with the proteasome, phagosome, and infection pathways. Overall, this study shows a basic and primary way for further functional research of the involvement of vitellogenin 2, alpha-2-macroglobulin-like protein, toll-like receptors (TLR-13), calpain, keratin-like proteins, and heat shock proteins against bacterial infection. Nonetheless, this first-ever comprehensive report of a proteomic sketch of EM from after infection provides systematic protein information to broadly understand the biological role of fish EM against bacterial infection.

Citing Articles

Proteomic map of the differentially expressed proteins in the skin of against infection.

Ali S, Dawar F, Ullah W, Hassan M, Ullah K, Zhao Z Fish Shellfish Immunol Rep. 2023; 5:100122.

PMID: 38023345 PMC: 10652109. DOI: 10.1016/j.fsirep.2023.100122.

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