Novel Image Analyser-assisted Morphometric Methodology Offer Unique Opportunity for Selection of Embryos with Potential for Implantation

Overview

Journal BMC Pregnancy Childbirth

Publisher Biomed Central

Specialty Gynecology & Obstetrics

Date 2023 Sep 28

PMID 37770819

Authors

Y Alhelou

M Hamdan

N Razali

Nam Adenan

J Ali

Affiliations

Soon will be listed here.

Abstract

Background: Previous studies looked into the connections between pregnancy and the Zona Pellucida (ZP) thickness and Zona Pellucida Thickness Variation (ZPTV), as well as the embryo's radius, circumference, perimeter and global symmetry. However, no research has linked embryo implantation and pregnancy to the percentage of ZP thinning, the reduction in ooplasm volume, and the increase in perivitelline space (PVS) volume. Our objective is to correlate the percentage of ZP thinning, the percentage of ooplasm volume shrinkage and the percentage of PVS increase to the implantation. These data will be used for embryo selection as well as it can be put into a software that will assist embryo selection.

Materials And Methods: Retrospective study included 281 patients, all of them had 2 embryos transferred, 149 patients got pregnant with two gestation sacs and 132 patients did not get pregnant. All of the transferred embryos had the ZP thickness measured several times from time of ICSI till Embryo Transfer (ET), the ooplasm volume was calculated from time of ICSI till two Pronuclei (2PN) fading and the PVS was calculated from the ICSI time till the 2PN fading.

Results: The first characteristic is the change in the average ZP thickness that decreased by 32.7% + 5.3% at 70 h for the implanted embryos (Group 1) versus 23.6% + 4.8% for non-implanted embryos (Group 2) p = 0.000. The second characteristic is the average reduction in the volume of the ooplasm which is 20.5% + 4.3% in Group 1 versus 15.1% + 5.2% in Group 2, p = 0.000. The third characteristic is the increase in the volume of the PVS which was 38.1% + 7.6% in Group 1 versus 31.6% + 9.7% in Group 2 p = 0.000.

Conclusion: The implanted embryos showed higher percent of ZP thinning, higher percent of ooplasm reduction and higher percent of PVS increase.

References

Scott L, Finn A, OLeary T, McLellan S, Hill J . Morphologic parameters of early cleavage-stage embryos that correlate with fetal development and delivery: prospective and applied data for increased pregnancy rates. Hum Reprod. 2006; 22(1):230-40. DOI: 10.1093/humrep/del358. View

Paternot G, Debrock S, De Neubourg D, DHooghe T, Spiessens C . Semi-automated morphometric analysis of human embryos can reveal correlations between total embryo volume and clinical pregnancy. Hum Reprod. 2013; 28(3):627-33. DOI: 10.1093/humrep/des427. View

Janny L, Menezo Y . Maternal age effect on early human embryonic development and blastocyst formation. Mol Reprod Dev. 1996; 45(1):31-7. DOI: 10.1002/(SICI)1098-2795(199609)45:1<31::AID-MRD4>3.0.CO;2-T. View

Hardarson T, Hanson C, Sjogren A, Lundin K . Human embryos with unevenly sized blastomeres have lower pregnancy and implantation rates: indications for aneuploidy and multinucleation. Hum Reprod. 2001; 16(2):313-8. DOI: 10.1093/humrep/16.2.313. View

Goyanes V, Costas E, Maneiro E . Morphometric categorization of the human oocyte and early conceptus. Hum Reprod. 1990; 5(5):613-8. DOI: 10.1093/oxfordjournals.humrep.a137155. View

Antczak M, Van Blerkom J . Temporal and spatial aspects of fragmentation in early human embryos: possible effects on developmental competence and association with the differential elimination of regulatory proteins from polarized domains. Hum Reprod. 1999; 14(2):429-47. DOI: 10.1093/humrep/14.2.429. View

Ziebe S, Petersen K, Lindenberg S, Andersen A, Gabrielsen A, Andersen A . Embryo morphology or cleavage stage: how to select the best embryos for transfer after in-vitro fertilization. Hum Reprod. 1997; 12(7):1545-9. DOI: 10.1093/humrep/12.7.1545. View

Nakahara T, Iwase A, Goto M, Harata T, Suzuki M, Ienaga M . Evaluation of the safety of time-lapse observations for human embryos. J Assist Reprod Genet. 2010; 27(2-3):93-6. PMC: 2842873. DOI: 10.1007/s10815-010-9385-8. View

Santos Filho E, Noble J, Wells D . A review on automatic analysis of human embryo microscope images. Open Biomed Eng J. 2011; 4:170-7. PMC: 3044885. DOI: 10.2174/1874120701004010170. View

10.

Bertrand E, Van den Bergh M, Englert Y . Does zona pellucida thickness influence the fertilization rate?. Hum Reprod. 1995; 10(5):1189-93. DOI: 10.1093/oxfordjournals.humrep.a136116. View

11.

Liu Y, Chapple V, Roberts P, Ali J, Matson P . Time-lapse videography of human oocytes following intracytoplasmic sperm injection: events up to the first cleavage division. Reprod Biol. 2014; 14(4):249-56. DOI: 10.1016/j.repbio.2014.08.003. View

12.

Pickering S, Taylor A, Johnson M, Braude P . An analysis of multinucleated blastomere formation in human embryos. Hum Reprod. 1995; 10(7):1912-22. DOI: 10.1093/oxfordjournals.humrep.a136206. View

13.

Beuchat A, Thevenaz P, Unser M, Ebner T, Senn A, Urner F . Quantitative morphometrical characterization of human pronuclear zygotes. Hum Reprod. 2008; 23(9):1983-92. DOI: 10.1093/humrep/den206. View

14.

Paternot G, Wetzels A, Thonon F, Vansteenbrugge A, Willemen D, Devroe J . Intra- and interobserver analysis in the morphological assessment of early stage embryos during an IVF procedure: a multicentre study. Reprod Biol Endocrinol. 2011; 9:127. PMC: 3181205. DOI: 10.1186/1477-7827-9-127. View

15.

Molina I, Martinez J, Pertusa J, Balasch S, Iniesta I, Pellicer A . Assessment of the implantation of day-2 human embryos by morphometric nonsubjective parameters. Fertil Steril. 2014; 102(4):1022-8. DOI: 10.1016/j.fertnstert.2014.06.026. View

16.

Palmstierna M, Murkes D, Csemiczky G, Andersson O, Wramsby H . Zona pellucida thickness variation and occurrence of visible mononucleated blastomers in preembryos are associated with a high pregnancy rate in IVF treatment. J Assist Reprod Genet. 1998; 15(2):70-5. PMC: 3455425. DOI: 10.1007/BF02766828. View

17.

Wright V, Chang J, Jeng G, Macaluso M . Assisted reproductive technology surveillance--United States, 2005. MMWR Surveill Summ. 2008; 57(5):1-23. View

18.

van Royen E, Mangelschots K, De Neubourg D, Laureys I, RYCKAERT G, Gerris J . Calculating the implantation potential of day 3 embryos in women younger than 38 years of age: a new model. Hum Reprod. 2001; 16(2):326-32. DOI: 10.1093/humrep/16.2.326. View

19.

Hunault C, Eijkemans M, Pieters M, Te Velde E, Habbema J, Fauser B . A prediction model for selecting patients undergoing in vitro fertilization for elective single embryo transfer. Fertil Steril. 2002; 77(4):725-32. DOI: 10.1016/s0015-0282(01)03243-5. View

20.

Wong C, Loewke K, Bossert N, Behr B, De Jonge C, Baer T . Non-invasive imaging of human embryos before embryonic genome activation predicts development to the blastocyst stage. Nat Biotechnol. 2010; 28(10):1115-21. DOI: 10.1038/nbt.1686. View