Method for Collection of Tear Fluid for Evaluation Its Antioxidant Properties

Overview

Journal Bull Exp Biol Med

Publisher Springer

Specialty General Medicine

Date 2023 Sep 28

PMID 37768462

Authors

V V Tyulina

I I Senin

Affiliations

Soon will be listed here.

Abstract

A technique of collection of the tear fluid with Schirmer strips for evaluation of the activity of the main antioxidant defense enzymes (superoxide dismutase, catalase, and glutathione peroxidase) in the tear fluid was proposed. The degree of extraction of the studied enzymes from the Schirmer strip is >85%. Cytometry showed that conjunctival and corneal cells can be transferred to the Schirmer strips during tear collection, which leads to sample contamination with intracellular fractions of the antioxidant enzymes. The approach proposed by us allows avoiding this contamination during tear fluid sampling. This technique makes it possible to increase the accuracy of determining the activity of antioxidant protection enzymes in the tear fluid and can be used for diagnostics of ocular surface pathologies in clinical practice.

References

Hsueh Y, Chen Y, Tsao Y, Cheng C, Wu W, Chen H . The Pathomechanism, Antioxidant Biomarkers, and Treatment of Oxidative Stress-Related Eye Diseases. Int J Mol Sci. 2022; 23(3). PMC: 8835903. DOI: 10.3390/ijms23031255. View

Chen Y, Mehta G, Vasiliou V . Antioxidant defenses in the ocular surface. Ocul Surf. 2009; 7(4):176-85. PMC: 4104792. DOI: 10.1016/s1542-0124(12)70185-4. View

Sacca S, Roszkowska A, Izzotti A . Environmental light and endogenous antioxidants as the main determinants of non-cancer ocular diseases. Mutat Res. 2013; 752(2):153-171. DOI: 10.1016/j.mrrev.2013.01.001. View

Behndig A, Svensson B, Marklund S, Karlsson K . Superoxide dismutase isoenzymes in the human eye. Invest Ophthalmol Vis Sci. 1998; 39(3):471-5. View

Sedlak L, Wojnar W, Zych M, Wygledowska-Promienska D . Influence of timolol, benzalkonium-preserved timolol, and benzalkonium-preserved brimonidine on oxidative stress biomarkers in the tear film. Cutan Ocul Toxicol. 2020; 39(3):260-268. DOI: 10.1080/15569527.2020.1787435. View

Gus P, Bello-Klein A, Llesuy S, Quinto G, Matos G, Bechara S . [Tear antioxidant potential in young adults]. Arq Bras Oftalmol. 2006; 69(4):565-70. DOI: 10.1590/s0004-27492006000400020. View

Lopez-Cisternas J, Castillo-Diaz J, Traipe-Castro L, Lopez-Solis R . A protein dye-binding assay on cellulose membranes for tear protein quantification: use of conventional schirmer strips. Cornea. 2007; 26(8):970-6. DOI: 10.1097/ICO.0b013e318093de64. View

Akkurt Arslan M, Kolman I, Pionneau C, Chardonnet S, Magny R, Baudouin C . Proteomic Analysis of Tears and Conjunctival Cells Collected with Schirmer Strips Using timsTOF Pro: Preanalytical Considerations. Metabolites. 2022; 12(1). PMC: 8778087. DOI: 10.3390/metabo12010002. View

VanDerMeid K, Su S, Krenzer K, Ward K, Zhang J . A method to extract cytokines and matrix metalloproteinases from Schirmer strips and analyze using Luminex. Mol Vis. 2011; 17:1056-63. PMC: 3086627. View

10.

Pieczynski J, Szulc U, Harazna J, Szulc A, Kiewisz J . Tear fluid collection methods: Review of current techniques. Eur J Ophthalmol. 2021; 31(5):2245-2251. DOI: 10.1177/1120672121998922. View

11.

Inaba T, Ohnishi-Kameyama M, Liu Y, Tanaka Y, Kobori M, Tamaki S . Quercetin improves lacrimal gland function through its anti-oxidant actions: Evidence from animal studies, and a pilot study in healthy human volunteers. Front Nutr. 2022; 9:974530. PMC: 9599396. DOI: 10.3389/fnut.2022.974530. View

12.

Kain R . Histopathology. Methods Mol Biol. 2016; 1508:185-193. DOI: 10.1007/978-1-4939-6515-1_9. View

13.

Small D, Hevy J, Tang-Liu D . Comparison of tear sampling techniques for pharmacokinetics analysis: ofloxacin concentrations in rabbit tears after sampling with schirmer tear strips, capillary tubes, or surgical sponges. J Ocul Pharmacol Ther. 2000; 16(5):439-46. DOI: 10.1089/jop.2000.16.439. View

14.

Sedlak L, Zych M, Wojnar W, Wygledowska-Promienska D . Effect of Topical Prostaglandin F2α Analogs on Selected Oxidative Stress Parameters in the Tear Film. Medicina (Kaunas). 2019; 55(7). PMC: 6681209. DOI: 10.3390/medicina55070366. View