The Viromes of Mosquitoes from the Natural Landscapes of Western Siberia

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2023 Sep 28

PMID 37766302

Authors

Vladimir A Ternovoi

Alexander N Shvalov

Mikhail Yu Kartashov

Eugenia P Ponomareva

Natalia L Tupota

Yuri A Khoroshavin

Roman B Bayandin

Anastasia V Gladysheva

Tamara P Mikryukova

Tatyana V Tregubchak

Valery B Loktev

Affiliations

Soon will be listed here.

Abstract

The metagenomic analysis of mosquitoes allows for the genetic characterization of mosquito-associated viruses in different regions of the world. This study applied a metagenomic approach to identify novel viral sequences in seven species of mosquitoes collected from the Novosibirsk region of western Siberia. Using NGS sequencing, we identified 15 coding-complete viral polyproteins (genomes) and 15 viral-like partial sequences in mosquitoes. The complete sequences for novel viruses or the partial sequences of capsid proteins, hypothetical viral proteins, and RdRps were used to identify their taxonomy. The novel viral sequences were classified within the orders and and the families , , , , , , and , with several attributed to four unclassified RNA viruses. Interestingly, the novel putative viruses and viral sequences were mainly associated with the mosquito . This study aimed to increase our understanding of the viral diversity in mosquitoes found in the natural habitats of Siberia, which is characterized by very long, snowy, and cold winters.

Citing Articles

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PMID: 39066322 PMC: 11281482. DOI: 10.3390/v16071160.

References

Hall-Mendelin S, McLean B, Bielefeldt-Ohmann H, Hobson-Peters J, Hall R, van den Hurk A . The insect-specific Palm Creek virus modulates West Nile virus infection in and transmission by Australian mosquitoes. Parasit Vectors. 2016; 9(1):414. PMC: 4960669. DOI: 10.1186/s13071-016-1683-2. View

Walker P, Firth C, Widen S, Blasdell K, Guzman H, Wood T . Evolution of genome size and complexity in the rhabdoviridae. PLoS Pathog. 2015; 11(2):e1004664. PMC: 4334499. DOI: 10.1371/journal.ppat.1004664. View

Attoui H, Mohd Jaafar F, Belhouchet M, Tao S, Chen B, Liang G . Liao ning virus, a new Chinese seadornavirus that replicates in transformed and embryonic mammalian cells. J Gen Virol. 2005; 87(Pt 1):199-208. DOI: 10.1099/vir.0.81294-0. View

Liu J, Carino E, Bera S, Gao F, May J, Simon A . Structural Analysis and Whole Genome Mapping of a New Type of Plant Virus Subviral RNA: Umbravirus-Like Associated RNAs. Viruses. 2021; 13(4). PMC: 8068935. DOI: 10.3390/v13040646. View

Marklewitz M, Zirkel F, Kurth A, Drosten C, Junglen S . Evolutionary and phenotypic analysis of live virus isolates suggests arthropod origin of a pathogenic RNA virus family. Proc Natl Acad Sci U S A. 2015; 112(24):7536-41. PMC: 4475995. DOI: 10.1073/pnas.1502036112. View

Xiao P, Han J, Zhang Y, Li C, Guo X, Wen S . Metagenomic Analysis of in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes From Dengue and Zika Viruses. Front Cell Infect Microbiol. 2018; 8:359. PMC: 6207848. DOI: 10.3389/fcimb.2018.00359. View

Hobson-Peters J, Yam A, Lu J, Setoh Y, May F, Kurucz N . A new insect-specific flavivirus from northern Australia suppresses replication of West Nile virus and Murray Valley encephalitis virus in co-infected mosquito cells. PLoS One. 2013; 8(2):e56534. PMC: 3584062. DOI: 10.1371/journal.pone.0056534. View

Xia H, Wang Y, Atoni E, Zhang B, Yuan Z . Mosquito-Associated Viruses in China. Virol Sin. 2018; 33(1):5-20. PMC: 5866263. DOI: 10.1007/s12250-018-0002-9. View

Rodgers M, Wilkinson E, Vallari A, McArthur C, Sthreshley L, Brennan C . Sensitive Next-Generation Sequencing Method Reveals Deep Genetic Diversity of HIV-1 in the Democratic Republic of the Congo. J Virol. 2017; 91(6). PMC: 5331799. DOI: 10.1128/JVI.01841-16. View

10.

Hall R, Bielefeldt-Ohmann H, McLean B, OBrien C, Colmant A, Piyasena T . Commensal Viruses of Mosquitoes: Host Restriction, Transmission, and Interaction with Arboviral Pathogens. Evol Bioinform Online. 2017; 12(Suppl 2):35-44. PMC: 5226260. DOI: 10.4137/EBO.S40740. View

11.

Nasar F, Erasmus J, Haddow A, Tesh R, Weaver S . Eilat virus induces both homologous and heterologous interference. Virology. 2015; 484:51-58. PMC: 4567418. DOI: 10.1016/j.virol.2015.05.009. View

12.

LaBeaud A, Bashir F, King C . Measuring the burden of arboviral diseases: the spectrum of morbidity and mortality from four prevalent infections. Popul Health Metr. 2011; 9(1):1. PMC: 3024945. DOI: 10.1186/1478-7954-9-1. View

13.

Li C, Shi M, Tian J, Lin X, Kang Y, Chen L . Unprecedented genomic diversity of RNA viruses in arthropods reveals the ancestry of negative-sense RNA viruses. Elife. 2015; 4. PMC: 4384744. DOI: 10.7554/eLife.05378. View

14.

Frey K, Biser T, Hamilton T, Santos C, Pimentel G, Mokashi V . Bioinformatic Characterization of Mosquito Viromes within the Eastern United States and Puerto Rico: Discovery of Novel Viruses. Evol Bioinform Online. 2016; 12(Suppl 2):1-12. PMC: 4912310. DOI: 10.4137/EBO.S38518. View

15.

Paraskevopoulou S, Kafer S, Zirkel F, Donath A, Petersen M, Liu S . Viromics of extant insect orders unveil the evolution of the flavi-like superfamily. Virus Evol. 2021; 7(1):veab030. PMC: 8129625. DOI: 10.1093/ve/veab030. View

16.

Messina J, Brady O, Scott T, Zou C, Pigott D, Duda K . Global spread of dengue virus types: mapping the 70 year history. Trends Microbiol. 2014; 22(3):138-46. PMC: 3946041. DOI: 10.1016/j.tim.2013.12.011. View

17.

Ding S, Howe J, Keese P, MacKenzie A, Meek D, Skotnicki M . The tymobox, a sequence shared by most tymoviruses: its use in molecular studies of tymoviruses. Nucleic Acids Res. 1990; 18(5):1181-7. PMC: 330433. DOI: 10.1093/nar/18.5.1181. View

18.

Presti R, Zhao G, Beatty W, Mihindukulasuriya K, Travassos da Rosa A, Popov V . Quaranfil, Johnston Atoll, and Lake Chad viruses are novel members of the family Orthomyxoviridae. J Virol. 2009; 83(22):11599-606. PMC: 2772707. DOI: 10.1128/JVI.00677-09. View

19.

Ternovoi V, Protopopova E, Kononova I, Olkhovikova E, Spiridonova E, Akopov G . [Cases of West Nile fever in Novosibirsk region in 2004, and the genotyping of its viral pathogen]. Vestn Ross Akad Med Nauk. 2007; (1):21-6. View

20.

Ohlund P, Hayer J, Lunden H, Hesson J, Blomstrom A . Viromics Reveal a Number of Novel RNA Viruses in Swedish Mosquitoes. Viruses. 2019; 11(11). PMC: 6893623. DOI: 10.3390/v11111027. View