Effects of Indonesian Shortfin Eel () By-Product Oil Supplementation on HOMA-IR and Lipid Profile in Obese Male Wistar Rats

Overview

Journal Nutrients

Date 2023 Sep 28

PMID 37764688

Authors

Ginna Megawati

Siti Shofiah Syahruddin

Winona Tjandra

Maya Kusumawati

Dewi Marhaeni Diah Herawati

Dida Achmad Gurnida

Ida Musfiroh

Affiliations

Soon will be listed here.

Abstract

The prevalence of people being overweight and obese has increased globally over the past decades. The use of omega-3 fatty acids-a compound usually primarily found in fish oil-has been known to improve the metabolic profile of obese patients. As the demand for eels increases, the number of waste products from the eels increases and creates environmental problems. This study was conducted to investigate the effect of a newly discovered Indonesian Shortfin eel by-product oil supplementation on the Homeostasis Model Assessment-Estimated Insulin Resistance (HOMA-IR) and lipid profiles of obese male (Lee index ≥ 0.3) Wistar rats (). The oil was extracted from waste products (heads). Fifteen obese rats were divided into three groups and were administered NaCl (C), commercial fish oil (CO), and Indonesian shortfin eel by-product oil (EO). All groups had statistically significant differences in total cholesterol, LDL, and triglyceride levels ( < 0.05). The CO and EO group showed a significant decrease in total cholesterol, LDL, and triglyceride after treatment. However, no significant difference was found in HDL levels and HOMA-IR. The supplementation of Indonesian shortfin eel by-product oil significantly improved lipid profile while effectively mitigating environmental challenges.

References

Belzung F, Raclot T, GROSCOLAS R . Fish oil n-3 fatty acids selectively limit the hypertrophy of abdominal fat depots in growing rats fed high-fat diets. Am J Physiol. 1993; 264(6 Pt 2):R1111-8. DOI: 10.1152/ajpregu.1993.264.6.R1111. View

Baillie R, Takada R, Nakamura M, Clarke S . Coordinate induction of peroxisomal acyl-CoA oxidase and UCP-3 by dietary fish oil: a mechanism for decreased body fat deposition. Prostaglandins Leukot Essent Fatty Acids. 1999; 60(5-6):351-6. DOI: 10.1016/s0952-3278(99)80011-8. View

Daidj N, Lamri-Senhadji M . Hepatoprotective and Anti-Obesity Properties of Sardine By-Product Oil in Rats Fed a High-Fat Diet. Prev Nutr Food Sci. 2021; 26(3):285-295. PMC: 8531423. DOI: 10.3746/pnf.2021.26.3.285. View

Kalupahana N, Claycombe K, Newman S, Stewart T, Siriwardhana N, Matthan N . Eicosapentaenoic acid prevents and reverses insulin resistance in high-fat diet-induced obese mice via modulation of adipose tissue inflammation. J Nutr. 2010; 140(11):1915-22. DOI: 10.3945/jn.110.125732. View

Garvey W, Mechanick J, Brett E, Garber A, Hurley D, Jastreboff A . AMERICAN ASSOCIATION OF CLINICAL ENDOCRINOLOGISTS AND AMERICAN COLLEGE OF ENDOCRINOLOGY COMPREHENSIVE CLINICAL PRACTICE GUIDELINES FOR MEDICAL CARE OF PATIENTS WITH OBESITY. Endocr Pract. 2016; 22 Suppl 3:1-203. DOI: 10.4158/EP161365.GL. View

Kontostathi M, Isou S, Mostratos D, Vasdekis V, Demertzis N, Kourounakis A . Influence of Omega-3 Fatty Acid-Rich Fish Oils on Hyperlipidemia: Effect of Eel, Sardine, Trout, and Cod Oils on Hyperlipidemic Mice. J Med Food. 2020; 24(7):749-755. PMC: 8311977. DOI: 10.1089/jmf.2020.0114. View

Lopes-Virella M, Stone P, Ellis S, Colwell J . Cholesterol determination in high-density lipoproteins separated by three different methods. Clin Chem. 1977; 23(5):882-4. View

Warnick G, Remaley A . Measurement of cholesterol in plasma and other body fluids. Curr Atheroscler Rep. 2001; 3(5):404-11. DOI: 10.1007/s11883-001-0079-7. View

Hainault I, Carolotti M, Hajduch E, Guichard C, LAVAU M . Fish oil in a high lard diet prevents obesity, hyperlipemia, and adipocyte insulin resistance in rats. Ann N Y Acad Sci. 1993; 683:98-101. DOI: 10.1111/j.1749-6632.1993.tb35696.x. View

10.

Gondim P, Rosa P, Okamura D, de Oliveira Silva V, Andrade E, Biihrer D . Benefits of Fish Oil Consumption over Other Sources of Lipids on Metabolic Parameters in Obese Rats. Nutrients. 2018; 10(1). PMC: 5793293. DOI: 10.3390/nu10010065. View

11.

Lionetti L, Mollica M, Sica R, Donizzetti I, Gifuni G, Pignalosa A . Differential effects of high-fish oil and high-lard diets on cells and cytokines involved in the inflammatory process in rat insulin-sensitive tissues. Int J Mol Sci. 2014; 15(2):3040-63. PMC: 3958898. DOI: 10.3390/ijms15023040. View

12.

Nauck M, Warnick G, Rifai N . Methods for measurement of LDL-cholesterol: a critical assessment of direct measurement by homogeneous assays versus calculation. Clin Chem. 2002; 48(2):236-54. View

13.

Nakatani T, Kim H, Kaburagi Y, Yasuda K, Ezaki O . A low fish oil inhibits SREBP-1 proteolytic cascade, while a high-fish-oil feeding decreases SREBP-1 mRNA in mice liver: relationship to anti-obesity. J Lipid Res. 2003; 44(2):369-79. DOI: 10.1194/jlr.M200289-JLR200. View

14.

Ruzickova J, Rossmeisl M, Prazak T, Flachs P, Sponarova J, Veck M . Omega-3 PUFA of marine origin limit diet-induced obesity in mice by reducing cellularity of adipose tissue. Lipids. 2005; 39(12):1177-85. DOI: 10.1007/s11745-004-1345-9. View

15.

Shibabaw T . Omega-3 polyunsaturated fatty acids: anti-inflammatory and anti-hypertriglyceridemia mechanisms in cardiovascular disease. Mol Cell Biochem. 2020; 476(2):993-1003. DOI: 10.1007/s11010-020-03965-7. View

16.

Raclot T, GROSCOLAS R, Langin D, Ferre P . Site-specific regulation of gene expression by n-3 polyunsaturated fatty acids in rat white adipose tissues. J Lipid Res. 1997; 38(10):1963-72. View

17.

Flachs P, Rossmeisl M, Bryhn M, Kopecky J . Cellular and molecular effects of n-3 polyunsaturated fatty acids on adipose tissue biology and metabolism. Clin Sci (Lond). 2008; 116(1):1-16. DOI: 10.1042/CS20070456. View

18.

Khan M, Hashim M, King J, Govender R, Mustafa H, Al Kaabi J . Epidemiology of Type 2 Diabetes - Global Burden of Disease and Forecasted Trends. J Epidemiol Glob Health. 2020; 10(1):107-111. PMC: 7310804. DOI: 10.2991/jegh.k.191028.001. View

19.

Ferre P . The biology of peroxisome proliferator-activated receptors: relationship with lipid metabolism and insulin sensitivity. Diabetes. 2004; 53 Suppl 1:S43-50. DOI: 10.2337/diabetes.53.2007.s43. View

20.

Pighin D, Karabatas L, Rossi A, Chicco A, Basabe J, Lombardo Y . Fish oil affects pancreatic fat storage, pyruvate dehydrogenase complex activity and insulin secretion in rats fed a sucrose-rich diet. J Nutr. 2003; 133(12):4095-101. DOI: 10.1093/jn/133.12.4095. View