Strain IPPBiotE33 Displays a Synergistic Effect with Bt185

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Sep 28

PMID 37762496

Authors

Liang Mi

Ziqiong Gu

Ying Li

Wenyue Xu

Changlong Shu

Jie Zhang

Xi Bai

Lili Geng

Affiliations

Soon will be listed here.

Abstract

The discovery and isolation of new non-Bt insecticidal bacteria and genes are significant for the development of new biopesticides against coleopteran pests. In this study, we evaluated the insecticidal activity of non-Bt insecticidal bacteria, PPBiotE33, IPPBiotC41, IPPBiotA42 and IPPBiotC43, isolated from the peanut rhizosphere. All these strains showed insecticidal activity against first- and third-instar larvae of , and . IPPBiotE33 showed the highest toxicity among the four strains and exhibited virulence against . The genome of IPPBiotE33 was sequenced, and a new protein, 03673, with growth inhibition effects on was obtained. In addition, IPPBiotE33 had a synergistic effect with Bt185 against in bioassays and back-inoculation experiments with peanut seedlings. IPPBiotE33 induced a decrease in hemocytes and an increase in phenol oxidase activity in hemolymph, known as the immunosuppressive effect, which mediated synergistic activity with Bt185. This study increased our knowledge of the new insecticidal strain IPPBiotE33 and shed new light on the research on new insecticidal coaction mechanisms and new blended pesticides.

Citing Articles

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PMID: 39119481 PMC: 11306962. DOI: 10.1515/biol-2022-0902.

References

Wang K, Shu C, Zhang J . Effective bacterial insecticidal proteins against coleopteran pests: A review. Arch Insect Biochem Physiol. 2019; 102(3):e21558. DOI: 10.1002/arch.21558. View

Tao K, Yu X, Liu Y, Shi G, Liu S, Hou T . Cloning, expression, and purification of insecticidal protein Pr596 from locust pathogen Serratia marcescens HR-3. Curr Microbiol. 2007; 55(3):228-33. DOI: 10.1007/s00284-007-0096-z. View

Chen L, Jiang H, Cheng Q, Chen J, Wu G, Kumar A . Enhanced nematicidal potential of the chitinase pachi from Pseudomonas aeruginosa in association with Cry21Aa. Sci Rep. 2015; 5:14395. PMC: 4585872. DOI: 10.1038/srep14395. View

Zhang X, Hu X, Li Y, Ding X, Yang Q, Sun Y . XaxAB-like binary toxin from Photorhabdus luminescens exhibits both insecticidal activity and cytotoxicity. FEMS Microbiol Lett. 2013; 350(1):48-56. DOI: 10.1111/1574-6968.12321. View

Morishita M, Masuda A, Mon H, Lee J, Kusakabe T, Tashiro K . Identification of an insecticidal toxin produced by Enterobacter sp. strain 532 isolated from diseased Bombyx mori silkworms. FEMS Microbiol Lett. 2019; 366(2). DOI: 10.1093/femsle/fny295. View

Shu C, Yan G, Wang R, Zhang J, Feng S, Huang D . Characterization of a novel cry8 gene specific to Melolonthidae pests: Holotrichia oblita and Holotrichia parallela. Appl Microbiol Biotechnol. 2009; 84(4):701-7. DOI: 10.1007/s00253-009-1971-2. View

Landsberg M, Jones S, Rothnagel R, Busby J, Marshall S, Simpson R . 3D structure of the Yersinia entomophaga toxin complex and implications for insecticidal activity. Proc Natl Acad Sci U S A. 2011; 108(51):20544-9. PMC: 3251104. DOI: 10.1073/pnas.1111155108. View

Wang M, Geng L, Xue B, Wang Z, Xu W, Shu C . Structure characteristics and function of a novel extracellular polysaccharide from Bacillus thuringiensis strain 4D19. Int J Biol Macromol. 2021; 189:956-964. DOI: 10.1016/j.ijbiomac.2021.08.193. View

Whitten M, Coates C . Re-evaluation of insect melanogenesis research: Views from the dark side. Pigment Cell Melanoma Res. 2017; 30(4):386-401. DOI: 10.1111/pcmr.12590. View

10.

Pechy-Tarr M, Bruck D, Maurhofer M, Fischer E, Vogne C, Henkels M . Molecular analysis of a novel gene cluster encoding an insect toxin in plant-associated strains of Pseudomonas fluorescens. Environ Microbiol. 2008; 10(9):2368-86. DOI: 10.1111/j.1462-2920.2008.01662.x. View

11.

Letunic I, Khedkar S, Bork P . SMART: recent updates, new developments and status in 2020. Nucleic Acids Res. 2020; 49(D1):D458-D460. PMC: 7778883. DOI: 10.1093/nar/gkaa937. View

12.

Mansfield S, Wilson M, Gerard P, Wilson D, Swaminathan J, Wright D . Potential for a biopesticide bait to control black beetle, Heteronychus arator (Coleoptera: Scarabaeidae). Pest Manag Sci. 2020; 76(12):4150-4158. DOI: 10.1002/ps.5973. View

13.

Xu W, Sun X, Mi L, Wang K, Gu Z, Wang M . Plants recruit insecticidal bacteria to defend against herbivore attacks. Microbiol Res. 2024; 281:127597. DOI: 10.1016/j.micres.2023.127597. View

14.

Yesilyurt A, Muratoglu H, Demirbag Z, Nalcacioglu R . Chilo iridescent virus encodes two functional metalloproteases. Arch Virol. 2018; 164(3):657-665. DOI: 10.1007/s00705-018-4108-z. View

15.

Zhang H, Teng X, Luo Q, Sheng Z, Guo X, Wang G . Flight and Walking Performance of Dark Black Chafer Beetle Holotrichia parallela (Coleoptera: Scarabaeidae) in the Presence of Known Hosts and Attractive Nonhost Plants. J Insect Sci. 2019; 19(2). PMC: 6415723. DOI: 10.1093/jisesa/iez019. View

16.

Virgillito C, Manica M, Marini G, Rosa R, Della Torre A, Martini S . Evaluation of Bacillus thuringiensis Subsp. Israelensis and Bacillus sphaericus Combination Against Culex pipiens in Highly Vegetated Ditches. J Am Mosq Control Assoc. 2022; 38(1):40-45. DOI: 10.2987/21-7024. View

17.

Ashburner M, Ball C, Blake J, Botstein D, Butler H, Cherry J . Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet. 2000; 25(1):25-9. PMC: 3037419. DOI: 10.1038/75556. View

18.

Caccia S, Di Lelio I, La Storia A, Marinelli A, Varricchio P, Franzetti E . Midgut microbiota and host immunocompetence underlie Bacillus thuringiensis killing mechanism. Proc Natl Acad Sci U S A. 2016; 113(34):9486-91. PMC: 5003288. DOI: 10.1073/pnas.1521741113. View

19.

Li Y, Zhao D, Wu H, Ji Y, Liu Z, Guo X . Bt GS57 Interaction With Gut Microbiota Accelerates Mortality. Front Microbiol. 2022; 13:835227. PMC: 8989089. DOI: 10.3389/fmicb.2022.835227. View

20.

Oliver J, Reding M, Youssef N, Klein M, Bishop B, Lewis P . Surface-applied insecticide treatments for quarantine control of Japanese beetle, Popillia japonica Newman (Coleoptera: Scarabaeidae), larvae in field-grown nursery plants. Pest Manag Sci. 2009; 65(4):381-90. DOI: 10.1002/ps.1701. View