Genotypes Distribution of Epstein-Barr Virus Among Lymphoma Patients in Ethiopia

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Sep 28

PMID 37762195

Authors

Seifegebriel Teshome

Elshafa Hassan Ahmed

Kidist Zealiyas

Abdulaziz Abubeker

Fisihatsion Tadesse

Christoph Weigel

Robert A Baiocchi

Tamrat Abebe

Affiliations

Soon will be listed here.

Abstract

Epstein-Barr virus (EBV) is an oncogenic herpes virus associated with several human malignancies. Two main EBV genotypes (type 1 and type 2) distinguished by the differences in EBV nuclear antigens are known. Geographic variability in these genetic differences has been observed in the incidence of some EBV-related tumors. Here, we investigated the genetic variation of EBV in lymphoma specimens collected in Ethiopia. A total of 207 DNA samples were used for EBV detection and typing, and and genes were used to detect and subtype the EBV genome, respectively. EBV genotype 1 was detected in 52.2% of lymphoma patients. EBV genotype 2 was detected in 38.2% of the lymphoma patients, and 9.7% were coinfected by both EBV genotypes. Overall, 52.8% of the Hodgkin's lymphoma (HL) patients and 51.8% of non-Hodgkin's lymphoma (NHL) patients showed the presence of genotype 1. Meanwhile, 42.8% and 2.3% of HL patients and 35.8% and 12.4% of NHL patients showed EBV genotype 2 and both genotypes, respectively. Significant associations between the age groups and EBV genotypes were observed ( = 0.027). However, no significant association was seen between EBV genotypes and other sociodemographic and clinical characteristics. This study showed that the distribution of EBV genotype 1 was higher in Ethiopian lymphoma patients.

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References

Pereira L, Franca E, Costa I, Lima I, Freire A, Ramos F . Epstein-Barr Virus (EBV) Genotypes Associated with the Immunopathological Profile of People Living with HIV-1: Immunological Aspects of Primary EBV Infection. Viruses. 2022; 14(2). PMC: 8880155. DOI: 10.3390/v14020168. View

Coleman C, Lang J, Sweet L, Smith N, Freed B, Pan Z . Epstein-Barr Virus Type 2 Infects T Cells and Induces B Cell Lymphomagenesis in Humanized Mice. J Virol. 2018; 92(21). PMC: 6189503. DOI: 10.1128/JVI.00813-18. View

Palser A, Grayson N, White R, Corton C, Correia S, Abdullah M . Genome diversity of Epstein-Barr virus from multiple tumor types and normal infection. J Virol. 2015; 89(10):5222-37. PMC: 4442510. DOI: 10.1128/JVI.03614-14. View

Wan Z, Chen Y, Hui J, Guo Y, Peng X, Wang M . Epstein-Barr virus variation in people living with human immunodeficiency virus in southeastern China. Virol J. 2023; 20(1):107. PMC: 10230783. DOI: 10.1186/s12985-023-02078-z. View

Wang Y, Ba Y, Chen Q, Han Y . [Clinical Significance of Peripheral Blood EBV-DNA Determination and Genotyping in Lymphoma Patients]. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2021; 29(6):1802-1806. DOI: 10.19746/j.cnki.issn.1009-2137.2021.06.019. View

Mechelli R, Manzari C, Policano C, Annese A, Picardi E, Umeton R . Epstein-Barr virus genetic variants are associated with multiple sclerosis. Neurology. 2015; 84(13):1362-8. PMC: 4388746. DOI: 10.1212/WNL.0000000000001420. View

Hui K, Chan T, Yang W, Shen J, Lam K, Kwok H . High risk Epstein-Barr virus variants characterized by distinct polymorphisms in the EBER locus are strongly associated with nasopharyngeal carcinoma. Int J Cancer. 2018; 144(12):3031-3042. DOI: 10.1002/ijc.32049. View

Santpere G, Darre F, Blanco S, Alcami A, Villoslada P, Alba M . Genome-wide analysis of wild-type Epstein-Barr virus genomes derived from healthy individuals of the 1,000 Genomes Project. Genome Biol Evol. 2014; 6(4):846-60. PMC: 4104767. DOI: 10.1093/gbe/evu054. View

Peng R, Han B, Cai Q, Zuo X, Xia T, Chen J . Genomic and transcriptomic landscapes of Epstein-Barr virus in extranodal natural killer T-cell lymphoma. Leukemia. 2018; 33(6):1451-1462. PMC: 6756073. DOI: 10.1038/s41375-018-0324-5. View

10.

Tzellos S, Farrell P . Epstein-barr virus sequence variation-biology and disease. Pathogens. 2014; 1(2):156-74. PMC: 4235690. DOI: 10.3390/pathogens1020156. View

11.

Yao Q, Tierney R, Croom-Carter D, Dukers D, Cooper G, Ellis C . Frequency of multiple Epstein-Barr virus infections in T-cell-immunocompromised individuals. J Virol. 1996; 70(8):4884-94. PMC: 190438. DOI: 10.1128/JVI.70.8.4884-4894.1996. View

12.

Chene A, Donati D, Guerreiro-Cacais A, Levitsky V, Chen Q, Falk K . A molecular link between malaria and Epstein-Barr virus reactivation. PLoS Pathog. 2007; 3(6):e80. PMC: 1891325. DOI: 10.1371/journal.ppat.0030080. View

13.

Chu P, Chang K, Chen W, Chen Y, Shibata D, Hayashi K . Epstein-Barr virus (EBV) nuclear antigen (EBNA)-4 mutation in EBV-associated malignancies in three different populations. Am J Pathol. 1999; 155(3):941-7. PMC: 1866909. DOI: 10.1016/S0002-9440(10)65193-0. View

14.

Ma S, Hegde S, Young K, Sullivan R, Rajesh D, Zhou Y . A new model of Epstein-Barr virus infection reveals an important role for early lytic viral protein expression in the development of lymphomas. J Virol. 2010; 85(1):165-77. PMC: 3014199. DOI: 10.1128/JVI.01512-10. View

15.

Mainou B, Raab-Traub N . LMP1 strain variants: biological and molecular properties. J Virol. 2006; 80(13):6458-68. PMC: 1488979. DOI: 10.1128/JVI.00135-06. View

16.

Montes-Mojarro I, Chen B, Ramirez-Ibarguen A, Quezada-Fiallos C, Perez-Baez W, Duenas D . Mutational profile and EBV strains of extranodal NK/T-cell lymphoma, nasal type in Latin America. Mod Pathol. 2019; 33(5):781-791. DOI: 10.1038/s41379-019-0415-5. View

17.

Maeda E, Akahane M, Kiryu S, Kato N, Yoshikawa T, Hayashi N . Spectrum of Epstein-Barr virus-related diseases: a pictorial review. Jpn J Radiol. 2009; 27(1):4-19. DOI: 10.1007/s11604-008-0291-2. View

18.

Trimeche M, Bonnet C, Korbi S, Boniver J, de Leval L . Association between Epstein-Barr virus and Hodgkin's lymphoma in Belgium: a pathological and virological study. Leuk Lymphoma. 2007; 48(7):1323-31. DOI: 10.1080/10428190701411177. View

19.

Tabibzadeh A, Karbalaie Niya M, Esghaei M, Bokharaei-Salim F, Ataei-Pirkooh A, Kiani S . Molecular Epidemiology of Epstein-Barr virus (EBV) in Patients with Hematologic Malignancies. Asian Pac J Cancer Prev. 2020; 21(3):693-698. PMC: 7437315. DOI: 10.31557/APJCP.2020.21.3.693. View

20.

Arvey A, Tempera I, Tsai K, Chen H, Tikhmyanova N, Klichinsky M . An atlas of the Epstein-Barr virus transcriptome and epigenome reveals host-virus regulatory interactions. Cell Host Microbe. 2012; 12(2):233-45. PMC: 3424516. DOI: 10.1016/j.chom.2012.06.008. View