Effect of Hydrogen Peroxide on Cyanobacterial Biofilms

Overview

Journal Antibiotics (Basel)

Specialty Pharmacology

Date 2023 Sep 28

PMID 37760746

Authors

Maria Joao Romeu

Joao Morais

Vitor Vasconcelos

Filipe Mergulhao

Affiliations

Soon will be listed here.

Abstract

Although a range of disinfecting formulations is commercially available, hydrogen peroxide is one of the safest chemical agents used for disinfection in aquatic environments. However, its effect on cyanobacterial biofilms is poorly investigated. In this work, biofilm formation by two filamentous cyanobacterial strains was evaluated over seven weeks on two surfaces commonly used in marine environments: glass and silicone-based paint (Sil-Ref) under controlled hydrodynamic conditions. After seven weeks, the biofilms were treated with a solution of hydrogen peroxide (HO) to assess if disinfection could affect long-term biofilm development. The cyanobacterial biofilms appeared to be tolerant to HO treatment, and two weeks after treatment, the biofilms that developed on glass by one of the strains presented higher biomass amounts than the untreated biofilms. This result emphasizes the need to correctly evaluate the efficiency of disinfection in cyanobacterial biofilms, including assessing the possible consequences of inefficient disinfection on the regrowth of these biofilms.

Citing Articles

Food Webs and Feedbacks: The Untold Ecological Relevance of Antimicrobial Resistance as Seen in Harmful Algal Blooms.

Banerji A, Brinkman N, Davis B, Franklin A, Jahne M, Keely S Microorganisms. 2024; 12(11).

PMID: 39597512 PMC: 11596618. DOI: 10.3390/microorganisms12112121.

References

Faria S, Teixeira-Santos R, Romeu M, Morais J, de Jong E, Sjollema J . Unveiling the Antifouling Performance of Different Marine Surfaces and Their Effect on the Development and Structure of Cyanobacterial Biofilms. Microorganisms. 2021; 9(5). PMC: 8161073. DOI: 10.3390/microorganisms9051102. View

Camps M, Barani A, Gregori G, Bouchez A, Le Berre B, Bressy C . Antifouling coatings influence both abundance and community structure of colonizing biofilms: a case study in the Northwestern Mediterranean Sea. Appl Environ Microbiol. 2014; 80(16):4821-31. PMC: 4135756. DOI: 10.1128/AEM.00948-14. View

Weenink E, Luimstra V, Schuurmans J, Herk M, Visser P, Matthijs H . Combatting cyanobacteria with hydrogen peroxide: a laboratory study on the consequences for phytoplankton community and diversity. Front Microbiol. 2015; 6:714. PMC: 4510418. DOI: 10.3389/fmicb.2015.00714. View

Piel T, Sandrini G, Muyzer G, Brussaard C, Slot P, Herk M . Resilience of Microbial Communities after Hydrogen Peroxide Treatment of a Eutrophic Lake to Suppress Harmful Cyanobacterial Blooms. Microorganisms. 2021; 9(7). PMC: 8304526. DOI: 10.3390/microorganisms9071495. View

Muhammad M, Idris A, Fan X, Guo Y, Yu Y, Jin X . Beyond Risk: Bacterial Biofilms and Their Regulating Approaches. Front Microbiol. 2020; 11:928. PMC: 7253578. DOI: 10.3389/fmicb.2020.00928. View

Sinha A, Eggleton M, Lochmann R . An environmentally friendly approach for mitigating cyanobacterial bloom and their toxins in hypereutrophic ponds: Potentiality of a newly developed granular hydrogen peroxide-based compound. Sci Total Environ. 2018; 637-638:524-537. DOI: 10.1016/j.scitotenv.2018.05.023. View

Daniel E, Weiss G, Murik O, Sukenik A, Lieman-Hurwitz J, Kaplan A . The response of Microcystis aeruginosa strain MGK to a single or two consecutive H O applications. Environ Microbiol Rep. 2019; 11(5):621-629. DOI: 10.1111/1758-2229.12789. View

Capita R, Riesco-Pelaez F, Alonso-Hernando A, Alonso-Calleja C . Exposure of Escherichia coli ATCC 12806 to sublethal concentrations of food-grade biocides influences its ability to form biofilm, resistance to antimicrobials, and ultrastructure. Appl Environ Microbiol. 2013; 80(4):1268-80. PMC: 3911067. DOI: 10.1128/AEM.02283-13. View

Fernandes S, Gomes I, Simoes M . Antimicrobial and antibiofilm potentiation by a triple combination of dual biocides and a phytochemical with complementary activity. Food Res Int. 2023; 167:112680. DOI: 10.1016/j.foodres.2023.112680. View

10.

Rossi F, De Philippis R . Role of cyanobacterial exopolysaccharides in phototrophic biofilms and in complex microbial mats. Life (Basel). 2015; 5(2):1218-38. PMC: 4500136. DOI: 10.3390/life5021218. View

11.

Blain S, Guillou J, Treguer P, Woerther P, Delauney L, Follenfant E . High frequency monitoring of the coastal marine environment using the MAREL buoy. J Environ Monit. 2004; 6(6):569-75. DOI: 10.1039/b314073c. View

12.

Zhou T, Cao H, Zheng J, Teng F, Wang X, Lou K . Suppression of water-bloom cyanobacterium Microcystis aeruginosa by algaecide hydrogen peroxide maximized through programmed cell death. J Hazard Mater. 2020; 393:122394. DOI: 10.1016/j.jhazmat.2020.122394. View

13.

Amin S, Parker M, Armbrust E . Interactions between diatoms and bacteria. Microbiol Mol Biol Rev. 2012; 76(3):667-84. PMC: 3429620. DOI: 10.1128/MMBR.00007-12. View

14.

Piel T, Sandrini G, White E, Xu T, Schuurmans J, Huisman J . Suppressing Cyanobacteria with Hydrogen Peroxide Is More Effective at High Light Intensities. Toxins (Basel). 2020; 12(1). PMC: 7020451. DOI: 10.3390/toxins12010018. View

15.

Romeu M, Rodrigues D, Azeredo J . Effect of sub-lethal chemical disinfection on the biofilm forming ability, resistance to antibiotics and expression of virulence genes of Enteritidis biofilm-surviving cells. Biofouling. 2020; 36(1):101-112. DOI: 10.1080/08927014.2020.1719077. View

16.

Hentschke G, Ramos V, Pinheiro A, Barreiro A, Costa M, Rego A . gen. nov., sp. nov., gen. nov., sp. nov. and sp. nov., isolated from inter- and subtidal environments from northern Portugal. Int J Syst Evol Microbiol. 2022; 72(10). DOI: 10.1099/ijsem.0.005552. View

17.

Romeu M, Alves P, Morais J, Miranda J, de Jong E, Sjollema J . Biofilm formation behaviour of marine filamentous cyanobacterial strains in controlled hydrodynamic conditions. Environ Microbiol. 2019; 21(11):4411-4424. DOI: 10.1111/1462-2920.14807. View

18.

Fan F, Shi X, Zhang M, Liu C, Chen K . Comparison of algal harvest and hydrogen peroxide treatment in mitigating cyanobacterial blooms via an in situ mesocosm experiment. Sci Total Environ. 2019; 694:133721. DOI: 10.1016/j.scitotenv.2019.133721. View

19.

Sukenik A, Kaplan A . Cyanobacterial Harmful Algal Blooms in Aquatic Ecosystems: A Comprehensive Outlook on Current and Emerging Mitigation and Control Approaches. Microorganisms. 2021; 9(7). PMC: 8306311. DOI: 10.3390/microorganisms9071472. View

20.

Ramos V, Morais J, Castelo-Branco R, Pinheiro A, Martins J, Regueiras A . Cyanobacterial diversity held in microbial biological resource centers as a biotechnological asset: the case study of the newly established LEGE culture collection. J Appl Phycol. 2018; 30(3):1437-1451. PMC: 5982461. DOI: 10.1007/s10811-017-1369-y. View