Antioxidant and Cytotoxic Potential of Extract and Bioactivity-Guided Isolation of Cytotoxic Components

Overview

Journal Antioxidants (Basel)

Date 2023 Sep 28

PMID 37760007

Authors

Ireneusz Sowa

Roman Paduch

Jaroslaw Moldoch

Dariusz Szczepanek

Jacek Szkutnik

Pawel Sowa

Katarzyna Tyszczuk-Rotko

Tomasz Blicharski

Magdalena Wojciak

Affiliations

Soon will be listed here.

Abstract

is a poorly understood plant in the context of biological activity, despite its widespread application in ethnomedicine in numerous European countries. The aim of this study was to assess the cytotoxic potential of the plant against human colorectal adenocarcinoma (HT29) and to isolate the plant components linked to this effect. Ultra-high performance liquid chromatography with a high-resolution/quadrupole time-of-flight mass spectrometer (UHPLC-HR/QTOF/MS-PDA) was used for the phytochemical characterization of the extract. Liquid-liquid extraction and preparative chromatography were employed for fractionation purposes. Our investigation demonstrated that the ethyl acetate fraction from showed significant cytotoxicity, and a bioactivity-guided approach led to the isolation of oxylipins, including traumatic acid, pinellic acid, and 9,10-dihydroxy-8-oxsooctadec-12-enic acid. The structures of the compounds were confirmed by nuclear magnetic resonance spectroscopy. Among these compounds, the last one exhibited significant cytotoxicity, though without selectivity, and traumatic acid was characterized by mild cytotoxicity. The cytotoxicity was linked to intracellular reactive oxygen species generation.

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