Development of a Novel Genetic Sexing Strain of Ceratitis Capitata Based on an X-autosome Translocation

Overview

Journal Sci Rep

Specialty Science

Date 2023 Sep 27

PMID 37758733

Authors

Carlos Caceres

Kostas Bourtzis

Georgia Gouvi

Marc J B Vreysen

Nanwintoum Severin Bimbile Somda

Martina Hejnickova

Frantisek Marec

Jose S Meza

Affiliations

Soon will be listed here.

Abstract

Genetic sexing strains (GSS), such as the Ceratitis capitata (medfly) VIENNA 8 strain, facilitate male-only releases and improve the efficiency and cost-effectiveness of sterile insect technique (SIT) applications. Laboratory domestication may reduce their genetic diversity and mating behaviour and hence, refreshment with wild genetic material is frequently needed. As wild males do not carry the T(Y;A) translocation, and wild females do not easily conform to artificial oviposition, the genetic refreshment of this GSS is a challenging and time-consuming process. In the present study, we report the development of a novel medfly GSS, which is based on a viable homozygous T(XX;AA) translocation using the same selectable markers, the white pupae and temperature-sensitive lethal genes. This allows the en masse cross of T(XX;AA) females with wild males, and the backcrossing of F males with the T(XX;AA) females thus facilitating the re-establishment of the GSS as well as its genetic refreshment. The rearing efficiency and mating competitiveness of the novel GSS are similar to those of the T(Y;A)-based VIENNA 8 GSS. However, its advantage to easily allow the genetic refreshment is of great importance as it can ensure the mass production of high-quality males and enhanced efficacy of operational SIT programs.

Citing Articles

On the impact of re-mating and residual fertility on the Sterile Insect Technique efficacy: Case study with the medfly, Ceratitis capitata.

Dumont Y, Oliva C PLoS Comput Biol. 2024; 20(5):e1012052.

PMID: 38709817 PMC: 11098522. DOI: 10.1371/journal.pcbi.1012052.

References

Isasawin S, Aketarawong N, Lertsiri S, Thanaphum S . Development of a genetic sexing strain in Bactrocera carambolae (Diptera: Tephritidae) by introgression of sex sorting components from B. dorsalis, Salaya1 strain. BMC Genet. 2014; 15 Suppl 2:S2. PMC: 4255791. DOI: 10.1186/1471-2156-15-S2-S2. View

Marchi A, Pili E . Ribosomal RNA genes in mosquitoes: localization by fluorescence in situ hybridization (FISH). Heredity (Edinb). 1994; 72 ( Pt 6):599-605. DOI: 10.1038/hdy.1994.83. View

Benet J, Oliver-Bonet M, Cifuentes P, Templado C, Navarro J . Segregation of chromosomes in sperm of reciprocal translocation carriers: a review. Cytogenet Genome Res. 2005; 111(3-4):281-90. DOI: 10.1159/000086901. View

Porras M, Meza J, Rajotte E, Bourtzis K, Caceres C . Improving the Phenotypic Properties of the Ceratitis capitata (Diptera: Tephritidae) Temperature-Sensitive Lethal Genetic Sexing Strain in Support of Sterile Insect Technique Applications. J Econ Entomol. 2020; 113(6):2688-2694. PMC: 7724746. DOI: 10.1093/jee/toaa220. View

Brianti M, Ananina G, Recco-Pimentel S, Klaczko L . Comparative analysis of the chromosomal positions of rDNA genes in species of the tripunctata radiation of Drosophila. Cytogenet Genome Res. 2009; 125(2):149-57. DOI: 10.1159/000227840. View

Goday C, Selivon D, Perondini A, Greciano P, Ruiz M . Cytological characterization of sex chromosomes and ribosomal DNA location in Anastrepha species (Diptera, Tephritidae). Cytogenet Genome Res. 2006; 114(1):70-6. DOI: 10.1159/000091931. View

Gourzi P, Gubb D, Livadaras Y, Caceres C, Franz G, Savakis C . The construction of the first balancer chromosome for the Mediterranean fruit fly, Ceratitis capitata. Mol Gen Genet. 2000; 264(1-2):127-36. DOI: 10.1007/s004380000294. View

Meza J, Bourtzis K, Zacharopoulou A, Gariou-Papalexiou A, Caceres C . Development and characterization of a pupal-colour based genetic sexing strain of Anastrepha fraterculus sp. 1 (Diptera: Tephritidae). BMC Genet. 2020; 21(Suppl 2):134. PMC: 7747363. DOI: 10.1186/s12863-020-00932-5. View

McKee B . Homologous pairing and chromosome dynamics in meiosis and mitosis. Biochim Biophys Acta. 2004; 1677(1-3):165-80. DOI: 10.1016/j.bbaexp.2003.11.017. View

10.

McKee B, Habera L, Vrana J . Evidence that intergenic spacer repeats of Drosophila melanogaster rRNA genes function as X-Y pairing sites in male meiosis, and a general model for achiasmatic pairing. Genetics. 1992; 132(2):529-44. PMC: 1205154. DOI: 10.1093/genetics/132.2.529. View

11.

Bolker B, Brooks M, Clark C, Geange S, Poulsen J, Stevens M . Generalized linear mixed models: a practical guide for ecology and evolution. Trends Ecol Evol. 2009; 24(3):127-35. DOI: 10.1016/j.tree.2008.10.008. View

12.

Ward C, Aumann R, Whitehead M, Nikolouli K, Leveque G, Gouvi G . White pupae phenotype of tephritids is caused by parallel mutations of a MFS transporter. Nat Commun. 2021; 12(1):491. PMC: 7820335. DOI: 10.1038/s41467-020-20680-5. View

13.

Rendon P, McInnis D, Lance D, Stewart J . Medfly (Diptera: Tephritidae) genetic sexing: large-scale field comparison of males-only and bisexual sterile fly releases in Guatemala. J Econ Entomol. 2004; 97(5):1547-53. DOI: 10.1603/0022-0493-97.5.1547. View

14.

Orozco D, Meza J, Zepeda S, Solis E, Quintero-Fong J . Tapachula-7, a new genetic sexing strain of the Mexican fruit fly (Diptera: Tephritidae): sexual compatibility and competitiveness. J Econ Entomol. 2013; 106(2):735-41. DOI: 10.1603/ec12441. View

15.

Zepeda-Cisneros C, Meza Hernandez J, Garcia-Martinez V, Ibanez-Palacios J, Zacharopoulou A, Franz G . Development, genetic and cytogenetic analyses of genetic sexing strains of the Mexican fruit fly, Anastrepha ludens Loew (Diptera: Tephritidae). BMC Genet. 2014; 15 Suppl 2:S1. PMC: 4255765. DOI: 10.1186/1471-2156-15-S2-S1. View

16.

Franz G . Recombination between homologous autosomes in medfly (Ceratitis capitata) males: type-1 recombination and the implications for the stability of genetic sexing strains. Genetica. 2002; 116(1):73-84. DOI: 10.1023/a:1020911725724. View

17.

Meza J, Haq I, Vreysen M, Bourtzis K, Kyritsis G, Caceres C . Comparison of classical and transgenic genetic sexing strains of Mediterranean fruit fly (Diptera: Tephritidae) for application of the sterile insect technique. PLoS One. 2018; 13(12):e0208880. PMC: 6294381. DOI: 10.1371/journal.pone.0208880. View

18.

Drosopoulou E, Nakou I, Sichova J, Kubickova S, Marec F, Mavragani-Tsipidou P . Sex chromosomes and associated rDNA form a heterochromatic network in the polytene nuclei of Bactrocera oleae (Diptera: Tephritidae). Genetica. 2012; 140(4-6):169-80. DOI: 10.1007/s10709-012-9668-3. View

19.

Whiting M . Phylogeny of the holometabolous insect orders based on 18S ribosomal DNA: when bad things happen to good data. EXS. 2002; (92):69-83. DOI: 10.1007/978-3-0348-8114-2_5. View

20.

Willhoeft U, Franz G . Identification of the sex-determining region of the Ceratitis capitata Y chromosome by deletion mapping. Genetics. 1996; 144(2):737-45. PMC: 1207564. DOI: 10.1093/genetics/144.2.737. View