Enhancing the Growth of Thermophilic Bacillus Licheniformis YYC4 by Low-intensity Fixed-frequency Continuous Ultrasound

Overview

Journal Ultrason Sonochem

Specialty Radiology

Date 2023 Sep 27

PMID 37757602

Authors

Chunhua Dai

Zhenzhen Shu

Xueting Xu

Pengfei Yan

Mokhtar Dabbour

Benjamin Kumah Mintah

Liurong Huang

Ronghai He

Haile Ma

Affiliations

Soon will be listed here.

Abstract

The effect of low-intensity fixed-frequency continuous ultrasound (LIFFCU) on the growth of Bacillus licheniformis YYC4 was investigated. The changes in morphology and activity of the organism, contributing to the growth were also explored. Compared with the control, a significant increase (48.95%) in the biomass of B. licheniformis YYC4 (at the logarithmic metaphase) was observed following the LIFFCU (28 kHz, 1.5 h and 120 W (equivalent to power density of 40 W/L)) treatment. SEM images showed that ultrasonication caused sonoporation, resulting in increased membrane permeability, evidenced by increase in cellular membrane potential, electrical conductivity of the culture, extracellular protein and nucleic acid, and intracellular Ca content. Furthermore, LIFFCU action remarkably increased the extracellular protease activity, volatile components of the culture medium, microbial metabolic activity, and spore germination of the strain. Therefore, LIFFCU could be used to efficiently promote the growth of targeted microorganisms.

References

Li W, Ma H, He R, Ren X, Zhou C . Prospects and application of ultrasound and magnetic fields in the fermentation of rare edible fungi. Ultrason Sonochem. 2021; 76:105613. PMC: 8207300. DOI: 10.1016/j.ultsonch.2021.105613. View

Rodriguez E, Seguer J, Rocabayera X, Manresa A . Cellular effects of monohydrochloride of L-arginine, N-lauroyl ethylester (LAE) on exposure to Salmonella typhimurium and Staphylococcus aureus. J Appl Microbiol. 2004; 96(5):903-12. DOI: 10.1111/j.1365-2672.2004.02207.x. View

Mitchell P . Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biol Rev Camb Philos Soc. 1966; 41(3):445-502. DOI: 10.1111/j.1469-185x.1966.tb01501.x. View

Rajasekhar P, Fan L, Nguyen T, Roddick F . Impact of sonication at 20 kHz on Microcystis aeruginosa, Anabaena circinalis and Chlorella sp. Water Res. 2011; 46(5):1473-81. DOI: 10.1016/j.watres.2011.11.017. View

Khanal S, Anand S, Muthukumarappan K . Evaluation of high-intensity ultrasonication for the inactivation of endospores of 3 bacillus species in nonfat milk. J Dairy Sci. 2014; 97(10):5952-63. DOI: 10.3168/jds.2014-7950. View

Ewe J, Wan Abdullah W, Bhat R, Karim A, Liong M . Enhanced growth of lactobacilli and bioconversion of isoflavones in biotin-supplemented soymilk upon ultrasound-treatment. Ultrason Sonochem. 2011; 19(1):160-73. DOI: 10.1016/j.ultsonch.2011.06.013. View

Ma H, Huang L, Jia J, He R, Luo L, Zhu W . Effect of energy-gathered ultrasound on Alcalase. Ultrason Sonochem. 2010; 18(1):419-24. DOI: 10.1016/j.ultsonch.2010.07.014. View

Yeo S, Liong M . Growth, bioconversion of isoflavones and probiotic properties of parent and subsequent passages of Lactobacillus upon ultraviolet radiation. Int J Food Sci Nutr. 2012; 63(7):821-31. DOI: 10.3109/09637486.2011.652942. View

Liu H, Hughes J, Rollins S, Chen B, Perkins E . Calcium entry via ORAI1 regulates glioblastoma cell proliferation and apoptosis. Exp Mol Pathol. 2011; 91(3):753-60. DOI: 10.1016/j.yexmp.2011.09.005. View

10.

Huang G, Tang Y, Sun L, Xing H, Ma H, He R . Ultrasonic irradiation of low intensity with a mode of sweeping frequency enhances the membrane permeability and cell growth rate of Candida tropicalis. Ultrason Sonochem. 2017; 37:518-528. DOI: 10.1016/j.ultsonch.2017.02.010. View

11.

Zhang Z, Xiong F, Wang Y, Dai C, Xing Z, Dabbour M . Fermentation of Saccharomyces cerevisiae in a one liter flask coupled with an external circulation ultrasonic irradiation slot: Influence of ultrasonic mode and frequency on the bacterial growth and metabolism yield. Ultrason Sonochem. 2019; 54:39-47. DOI: 10.1016/j.ultsonch.2019.02.017. View

12.

Sinisterra J . Application of ultrasound to biotechnology: an overview. Ultrasonics. 1992; 30(3):180-5. DOI: 10.1016/0041-624x(92)90070-3. View

13.

Dahroud B, Mokarram R, Khiabani M, Hamishehkar H, Zahedi Bialvaei A, Yousefi M . Low intensity ultrasound increases the fermentation efficiency of Lactobacillus casei subsp.casei ATTC 39392. Int J Biol Macromol. 2016; 86:462-7. DOI: 10.1016/j.ijbiomac.2016.01.103. View

14.

Ojha K, Burgess C, Duffy G, Kerry J, Tiwari B . Integrated phenotypic-genotypic approach to understand the influence of ultrasound on metabolic response of Lactobacillus sakei. PLoS One. 2018; 13(1):e0191053. PMC: 5784923. DOI: 10.1371/journal.pone.0191053. View

15.

Kanazawa A, Ikeda T, Endo T . A novel approach to mode of action of cationic biocides: morphological effect on antibacterial activity. J Appl Bacteriol. 1995; 78(1):55-60. DOI: 10.1111/j.1365-2672.1995.tb01673.x. View

16.

Dai C, Hou Y, Xu H, Huang L, Dabbour M, Mintah B . Effect of solid-state fermentation by three different Bacillus species on composition and protein structure of soybean meal. J Sci Food Agric. 2021; 102(2):557-566. DOI: 10.1002/jsfa.11384. View

17.

Nyborg W . Ultrasonic microstreaming and related phenomena. Br J Cancer Suppl. 1982; 5:156-60. PMC: 2149335. View

18.

Zhang H, Ma H, Liu W, Pei J, Wang Z, Zhou H . Ultrasound enhanced production and antioxidant activity of polysaccharides from mycelial fermentation of Phellinus igniarius. Carbohydr Polym. 2014; 113:380-7. DOI: 10.1016/j.carbpol.2014.07.027. View

19.

Guo D, Du Y, Wu Q, Jiang W, Bi H . Disrupted calcium homeostasis is involved in elevated zinc ion-induced photoreceptor cell death. Arch Biochem Biophys. 2014; 560:44-51. DOI: 10.1016/j.abb.2014.07.014. View

20.

Wei Q, Yao J, Chen R, Yang S, Tang Y, Ma X . Low-frequency ultrasound and nitrogen limitation induced enhancement in biomass production and lipid accumulation of Tetradesmus obliquus FACHB-12. Bioresour Technol. 2022; 358:127387. DOI: 10.1016/j.biortech.2022.127387. View