Improving Cryopreservation Efficiency and Pregnancy Rate Through Superovulation with Follicle-Stimulating Hormone in Korean Hanwoo Cows Via Ovum Pick Up

Overview

Journal Vet Sci

Publisher MDPI

Specialty Veterinary Medicine

Date 2023 Sep 27

PMID 37756101

Authors

Daehyun Kim

Junkoo Yi

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to devise an efficient technique for generating embryos from high-quality bovine females. Oocytes were collected from 20 control and 15 Hanwoo () females treated with the FSH. A combination of decreasing FSH doses (36, 36, 24, and 24 mg, 12 h apart), progesterone, estrogen, and prostaglandins were administered to synchronize and mildly stimulate the animals. The FSH-treated group (1125 oocytes) and control group (1022 oocytes) exhibited a higher proportion of grade A and B oocytes (88.2%) than the other grades ( < 0.05), with most at the germinal vesicle 2 stage (64.0%). Moreover, the FSH-treated group achieved a notably higher blastocyst rate (44.7%) compared to the control group (31.1%) ( < 0.05). After undergoing vitrification and in vitro culture (IVC) warming, embryos in the FSH group exhibited higher re-expansion rates (grade 1: 86.9%; grades 2 and 3: 57.9%) compared to those in the control ( < 0.05). This highlights the positive impact of FSH treatment on in vitro embryo production (IVEP) and the OPU rate.

Citing Articles

Conception rate according to the size and location of corpus luteum and coexistent follicle before embryo transfer in Hanwoo.

Lim S, Yi J, Ha J, Park J, Kwon W, Yu D J Anim Sci Technol. 2024; 66(6):1127-1136.

PMID: 39691622 PMC: 11647416. DOI: 10.5187/jast.2024.e31.

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