Effect of the Probiotic DE-CA9 on Fecal Scores, Serum Oxidative Stress Markers and Fecal and Serum Metabolome in Healthy Dogs

Overview

Journal Vet Sci

Publisher MDPI

Specialty Veterinary Medicine

Date 2023 Sep 27

PMID 37756088

Authors

Karin Allenspach

Chi-Hsuan Sung

Jose Joaquin Ceron

Camila Peres Rubio

Agnes Bourgois-Mochel

Jan S Suchodolski

Lingnan Yuan

Debosmita Kundu

Joan Colom Comas

Kieran Rea

Jonathan P Mochel

Affiliations

Soon will be listed here.

Abstract

Background: There is increasing interest in the use of Bacillus species as probiotics since their spore-forming ability favors their survival in the acidic gastric environment over other probiotic species. The subsequent germination of to their vegetative form allows for their growth in the small intestine and may increase their beneficial effect on the host. strains have also previously been shown to have beneficial effects in humans and production animals, however, no reports are available so far on their use in companion animals.

Study Design: The goal of this study was therefore to investigate the daily administration of 1 × 10 cfu DE-CA9 orally per day versus placebo on health parameters, fecal scores, fecal microbiome, fecal metabolomics, as well as serum metabolomics and oxidative stress markers in ten healthy Beagle dogs in a parallel, randomized, prospective, placebo-controlled design over a period of 45 days.

Results: DE-CA9 decreased the oxidative status compared to controls for advanced oxidation protein products (AOPP), thiobarbituric acid reactive substances (TBARS) and reactive oxygen metabolites (d-ROMS), suggesting an antioxidant effect of the treatment. Fecal metabolomics revealed a significant reduction in metabolites associated with tryptophan metabolism in the DE-CA9-treated group. DE-CA9 also significantly decreased phenylalanine and homocysteine and increased homoserine and threonine levels. Amino acid metabolism was also affected in the serum metabolome, with increased levels of urea and cadaverine, and reductions in -acetylornithine in DE-CA9 compared to controls. Similarly, changes in essential amino acids were observed, with a significant increase in tryptophan and lysine levels and a decrease in homocysteine. An increase in serum guanine and deoxyuridine was also detected, with a decrease in beta-alanine in the animals that ingested DE-CA9.

Conclusions: Data generated throughout this study suggest that the daily administration of 1 × 10 cfu of DE-CA9 in healthy Beagle dogs is safe and does not affect markers of general health and fecal scores. Furthermore, DE-CA9 administration had a potential positive effect on some serum markers of oxidative stress, and protein and lipid metabolism in serum and feces.

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