The HOS1-PIF4/5 Module Controls Callus Formation in Leaf Explants

Overview

Journal Plant Signal Behav

Specialty Biology

Date 2023 Sep 25

PMID 37747842

Authors

Kyounghee Lee

Dohee Koo

Ok-Sun Park

Pil Joon Seo

Affiliations

Soon will be listed here.

Abstract

A two-step plant regeneration has been widely exploited to genetic manipulation and genome engineering in plants. Despite technical importance, understanding of molecular mechanism underlying plant regeneration remains to be fully elucidated. Here, we found that the HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 1 (HOS1)-PHYTOCHROME INTERACTING FACTOR 4/5 (PIF4/5) module participates in callus formation. Consistent with the repressive role of HOS1 in PIF transcriptional activation activity, mutant leaf explants exhibited enhanced callus formation, whereas mutant leaf explants showed reduced callus size. The HOS1-PIF4/5 function would be largely dependent on auxin biosynthesis and signaling, which are essential for callus initiation and proliferation. Our findings suggest that the HOS1-PIF4/5 module plays a pivotal role in auxin-dependent callus formation in .

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