Differential Expression of MiR-146 and MiR-155 in Active and Latent Tuberculosis Infection

Overview

Journal Iran J Public Health

Date 2023 Sep 25

PMID 37744552

Authors

Ebrahim Alijani

Farhad Riazi Rad

Asal Katebi

Soheila Ajdary

Affiliations

Soon will be listed here.

Abstract

Background: Tuberculosis (TB) is one of the leading causes of death worldwide. Besides, one-third of the world population is infected with (MTB) while staying clinically asymptomatic; the situation is called latent TB infection (LTBI). MiR-21, miR-31, miR-146a, and miR-155 play an important role in many immune and inflammatory pathways. In the present study the expression levels of MiR-21, miR-31, miR-146a, and miR-155 in peripheral blood mononuclear cells (PBMCs) from patients with active TB, latently infected individuals (LTBI), and healthy controls (HC) were investigated. Participants were recruited at the Bouali Hospital, Zahedan University of Medical Sciences, Zahedan, Iran from 2010 to 2011.

Methods: PBMCs were stimulated with PPD before RNA extraction. TaqMan RT-qPCR assay was used to analyze the expression levels of miRNAs.

Results: The results indicated no significant differences in the expression of miR-21 and miR-31 between different groups; however, in patients with active TB, the expression of miR-21 (=0.03) and miR-31 (=0.04) were significantly increased after stimulation with PPD compared to the unstimulated condition. The expression of miR-146 in response to PPD in both LTBI (=0.02) and TB (=0.03) groups compared to the HC group was increased. No significant differences were found in the expression level of miR-155 in response to PPD between LTBI and HC groups. However, the fold change was significantly higher in the TB group in comparison with the HC (=0.03) and LTBI (=0.05) groups.

Conclusion: The results confirm the main role of miR-146 and miR-155 in TB infection and suggest a role for miR-146 and miR-155 as infection and activation markers in tuberculosis infection, respectively.

Citing Articles

The role of exosomal miRNAs in host pathogen cross-talk as diagnostic and therapeutic biomarkers.

Mukhtar F, Guarnieri A, Brancazio N, Falcone M, Di Naro M, Azeem M Front Microbiol. 2024; 15:1441781.

PMID: 39176271 PMC: 11340542. DOI: 10.3389/fmicb.2024.1441781.

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