Effects of Volatile Anesthetics on Circadian Rhythm in Mice: a Comparative Study of Sevoflurane, Desflurane, and Isoflurane

Overview

Journal J Anesth

Specialty Anesthesiology

Date 2023 Sep 23

PMID 37741919

Authors

Sho Sugimura

Ryo Imai

Takasumi Katoh

Hiroshi Makino

Kazuya Hokamura

Tadayoshi Kurita

Yasuhito Suzuki

Yoshitaka Aoki

Tetsuro Kimura

Kazuo Umemura

Yoshiki Nakajima

Affiliations

Soon will be listed here.

Abstract

Purpose: Volatile anesthetics affect the circadian rhythm of mammals, although the effects of different types of anesthetics are unclear. Here, we anesthetized mice using several volatile anesthetics at two different times during the day. Our objective was to compare the effects of these anesthetics on circadian rhythm.

Methods: Male adult C57BL/6 J mice were divided into eight groups (n = 8 each) based on the anesthetic (sevoflurane, desflurane, isoflurane, or no anesthesia) and anesthesia time (Zeitgeber time [ZT] 6-12 or ZT18-24). Mice were anesthetized for 6 h using a 0.5 minimum alveolar concentration (MAC) dose under constant dark conditions. The difference between the start of the active phase before and after anesthesia was measured as a phase shift. Clock genes were measured by polymerase chain reaction in suprachiasmatic nucleus (SCN) samples removed from mouse brain after anesthesia (n = 8-9 each).

Results: Phase shift after anesthesia at ZT6-12 using sevoflurane (- 0.49 h) was smaller compared with desflurane (- 1.1 h) and isoflurane (- 1.4 h) (p < 0.05). Clock mRNA (ZT6-12, p < 0.05) and Per2 mRNA (ZT18-24, p < 0.05) expression were different between the groups after anesthesia.

Conclusion: 0.5 MAC sevoflurane anesthesia administered during the late inactive to early active phase has less impact on the phase shift of circadian rhythm than desflurane and isoflurane. This may be due to differences in the effects of volatile anesthetics on the expression of clock genes in the SCN, the master clock of the circadian rhythm.

Citing Articles

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PMID: 37558835 DOI: 10.1007/s00540-023-03226-z.

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