Mapping the Cellular and Molecular Landscape of Cardiac Non-myocytes in Murine Diabetic Cardiomyopathy

Overview

Journal iScience

Publisher Cell Press

Date 2023 Sep 22

PMID 37736052

Authors

Charles D Cohen

Miles J De Blasio

Gabriella E Farrugia

Malathi S I Dona

Ian Hsu

Darnel Prakoso

Helen Kiriazis

Crisdion Krstevski

David M Nash

Mandy Li

Taylah L Gaynor

Minh Deo

Grant R Drummond

Rebecca H Ritchie

Alexander R Pinto

Affiliations

Soon will be listed here.

Abstract

Diabetes is associated with a significantly elevated risk of heart failure. However, despite extensive efforts to characterize the phenotype of the diabetic heart, the molecular and cellular protagonists that underpin cardiac pathological remodeling in diabetes remain unclear, with a notable paucity of data regarding the impact of diabetes on non-myocytes within the heart. Here we aimed to define key differences in cardiac non-myocytes between spontaneously type-2 diabetic () and healthy control mouse hearts. Single-cell transcriptomic analysis revealed a concerted diabetes-induced cellular response contributing to cardiac remodeling. These included cell-specific activation of gene programs relating to fibroblast hyperplasia and cell migration, and dysregulation of pathways involving vascular homeostasis and protein folding. This work offers a new perspective for understanding the cellular mediators of diabetes-induced cardiac pathology, and pathways that may be targeted to address the cardiac complications associated with diabetes.

Citing Articles

Integrative Single-Cell Analysis of Cardiomyopathy Identifies Differences in Cell Stemness and Transcriptional Regulatory Networks among Fibroblast Subpopulations.

Nie W, Zhao Z, Liu Y, Wang Y, Zhang J, Hu Y Cardiol Res Pract. 2024; 2024:3131633.

PMID: 38799173 PMC: 11127766. DOI: 10.1155/2024/3131633.

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