A DNA Nanodevice for Mapping Sodium at Single-organelle Resolution

Overview

Journal Nat Biotechnol

Specialty Biotechnology

Date 2023 Sep 21

PMID 37735265

Authors

Junyi Zou

Koushambi Mitra

Palapuravan Anees

Daphne Oettinger

Joseph R Ramirez

Aneesh Tazhe Veetil

Priyanka Dutta Gupta

Rajini Rao

Jayson J Smith

Paschalis Kratsios

Yamuna Krishnan

Affiliations

Soon will be listed here.

Abstract

Cellular sodium ion (Na) homeostasis is integral to organism physiology. Our current understanding of Na homeostasis is largely limited to Na transport at the plasma membrane. Organelles may also contribute to Na homeostasis; however, the direction of Na flow across organelle membranes is unknown because organellar Na cannot be imaged. Here we report a pH-independent, organelle-targetable, ratiometric probe that reports lumenal Na. It is a DNA nanodevice containing a Na-sensitive fluorophore, a reference dye and an organelle-targeting domain. By measuring Na at single endosome resolution in mammalian cells and Caenorhabditis elegans, we discovered that lumenal Na levels in each stage of the endolysosomal pathway exceed cytosolic levels and decrease as endosomes mature. Further, we find that lysosomal Na levels in nematodes are modulated by the Na/H exchanger NHX-5 in response to salt stress. The ability to image subcellular Na will unveil mechanisms of Na homeostasis at an increased level of cellular detail.

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