Comprehensive Analysis of Chemokine Gene Polymorphisms in Korean Children with Autoimmune Thyroid Disease

Overview

Journal Sci Rep

Specialty Science

Date 2023 Sep 20

PMID 37730733

Authors

Chungwoo Shin

In-Cheol Baek

Won Kyoung Cho

Tai-Gyu Kim

Byung-Kyu Suh

Affiliations

Soon will be listed here.

Abstract

Chemokines are chemotactic cytokines that can cause directed migration of leukocytes. The aim of this study was to examine differences in single nucleotide polymorphisms (SNP) of chemokine in AITD patients compared to normal controls. A total of 86 Korean pediatric patients were included in the patient group and 183 adults were included in the normal control group. To compare influences of several chemokine gene polymorphisms, 25 SNPs in 16 chemokine genes were analyzed. Genotype frequencies of CCL11(rs3744508)AA(OR = 6.9) and CCR2(rs1799864)AA(OR = 3.8) were higher in the AITD patients than in the controls, whereas CCL17(rs223828)CC was lower in the AITD patients than in the controls(OR = 0.4). In comparison between Graves' disease (GD) patients and controls, genotype frequency of CCL17(rs223828)CC(OR = 0.4) was lower in the GD group, whereas those of CCR2(rs1799864)AA(OR = 4.8) were higher in the GD group. The genotype frequency of CCL11(rs3744508)AA(OR = 11.3) was higher in Hashimoto's thyroiditis (HT) patients, whereas that of CXCL8(rs2227306)CC(OR = 0.4) was lower in HT patients. Polymorphisms of CCL11(rs3744508), CCL17(rs223828), and CCR2(rs1799864) might be associated with AITD, with CCL17(rs223828), CCR2(rs1799864) and CXCR2(rs2230054, rs1126579) affecting GD and CCL11(rs3744508) and CXCL8(rs2227306) affecting HT in Korean children.

References

Chowdhury P, Khan S . Significance of CCL2, CCL5 and CCR2 polymorphisms for adverse prognosis of Japanese encephalitis from an endemic population of India. Sci Rep. 2017; 7(1):13716. PMC: 5651904. DOI: 10.1038/s41598-017-14091-8. View

Qi Y, Li X, Zhang Q, Huang F, Lin D, Zhou Y . Increased chemokine (C-C motif) ligand 21 expression and its correlation with osteopontin in Graves' disease. Endocrine. 2015; 50(1):123-9. DOI: 10.1007/s12020-015-0552-7. View

Ye Y, Yang X, Long B, Pang H, Zhu Y, Zhang S . Association Between a CCL17 Genetic Variant and Risk of Coronary Artery Disease in a Chinese Han Population. Circ J. 2017; 82(1):224-231. DOI: 10.1253/circj.CJ-17-0190. View

Kwon H, Jung J, Han K, Park Y, Cho J, Lee D . Prevalence and Annual Incidence of Thyroid Disease in Korea from 2006 to 2015: A Nationwide Population-Based Cohort Study. Endocrinol Metab (Seoul). 2018; 33(2):260-267. PMC: 6021312. DOI: 10.3803/EnM.2018.33.2.260. View

Zhang Z, Feng J, Mao A, Le K, La Placa D, Wu X . SNPs in inflammatory genes CCL11, CCL4 and MEFV in a fibromyalgia family study. PLoS One. 2018; 13(6):e0198625. PMC: 6013222. DOI: 10.1371/journal.pone.0198625. View

Weetman A . Cellular immune responses in autoimmune thyroid disease. Clin Endocrinol (Oxf). 2004; 61(4):405-13. DOI: 10.1111/j.1365-2265.2004.02085.x. View

Zhao H, Xu Y, Cui J . CXCR2 (rs3890158 and rs2230054) and CXCL4 (rs352008 and rs1801572) gene polymorphisms in patients with thoracic aortic aneurysm. Biotechnol Genet Eng Rev. 2023; 40(4):4300-4312. DOI: 10.1080/02648725.2023.2210011. View

Antonelli A, Ferrari S, Corrado A, Ferrannini E, Fallahi P . Increase of interferon-γ inducible CXCL9 and CXCL11 serum levels in patients with active Graves' disease and modulation by methimazole therapy. Thyroid. 2013; 23(11):1461-9. DOI: 10.1089/thy.2012.0485. View

. Classification of eye changes of Graves' disease. Thyroid. 1992; 2(3):235-6. DOI: 10.1089/thy.1992.2.235. View

10.

Menyhart O, Weltz B, Gyorffy B . MultipleTesting.com: A tool for life science researchers for multiple hypothesis testing correction. PLoS One. 2021; 16(6):e0245824. PMC: 8189492. DOI: 10.1371/journal.pone.0245824. View

11.

Romagnani P, Rotondi M, Lazzeri E, Lasagni L, Francalanci M, Buonamano A . Expression of IP-10/CXCL10 and MIG/CXCL9 in the thyroid and increased levels of IP-10/CXCL10 in the serum of patients with recent-onset Graves' disease. Am J Pathol. 2002; 161(1):195-206. PMC: 1850693. DOI: 10.1016/S0002-9440(10)64171-5. View

12.

Hu D, Wang H, Huang X, Jiang Y, Qin Y, Xiong B . Investigation of association between IL-8 serum levels and IL8 polymorphisms in Chinese patients with sepsis. Gene. 2016; 594(1):165-170. DOI: 10.1016/j.gene.2016.09.024. View

13.

Antonelli A, Rotondi M, Ferrari S, Fallahi P, Romagnani P, Sellari Franceschini S . Interferon-gamma-inducible alpha-chemokine CXCL10 involvement in Graves' ophthalmopathy: modulation by peroxisome proliferator-activated receptor-gamma agonists. J Clin Endocrinol Metab. 2005; 91(2):614-20. DOI: 10.1210/jc.2005-1689. View

14.

Zang F, Yue M, Zhuo L, Wu J, Liu M, Yao Y . Association of CXCR2 genotype variations with HCV clearance in a Chinese population. Arch Virol. 2018; 163(10):2711-2718. DOI: 10.1007/s00705-018-3872-0. View

15.

Antonelli A, Ferrari S, Frascerra S, Pupilli C, Mancusi C, Metelli M . CXCL9 and CXCL11 chemokines modulation by peroxisome proliferator-activated receptor-alpha agonists secretion in Graves' and normal thyrocytes. J Clin Endocrinol Metab. 2010; 95(12):E413-20. DOI: 10.1210/jc.2010-0923. View

16.

Frecker M, STENSZKY V, Balazs C, Kozma L, Kraszits E, Farid N . Genetic factors in Graves' ophthalmopathy. Clin Endocrinol (Oxf). 1986; 25(5):479-85. DOI: 10.1111/j.1365-2265.1986.tb03599.x. View

17.

Yin X, Sachidanandam R, Morshed S, Latif R, Shi R, Davies T . mRNA-Seq reveals novel molecular mechanisms and a robust fingerprint in Graves' disease. J Clin Endocrinol Metab. 2014; 99(10):E2076-83. PMC: 4184074. DOI: 10.1210/jc.2014-1735. View

18.

Ashhab Y, Dominguez O, Sospedra M, Lucas-Martin A, Pujol-Borrell R . A one-tube polymerase chain reaction protocol demonstrates CC chemokine overexpression in Graves' disease glands. J Clin Endocrinol Metab. 1999; 84(8):2873-82. DOI: 10.1210/jcem.84.8.5909. View

19.

Baek I, Choi E, Shin D, Kim H, Choi H, Kim T . Distributions of HLA-A, -B, and -DRB1 alleles typed by amplicon-based next generation sequencing in Korean volunteer donors for unrelated hematopoietic stem cell transplantation. HLA. 2020; 97(2):112-126. DOI: 10.1111/tan.14134. View

20.

Sokol C, Luster A . The chemokine system in innate immunity. Cold Spring Harb Perspect Biol. 2015; 7(5). PMC: 4448619. DOI: 10.1101/cshperspect.a016303. View