Single-nucleus Transcriptomic Mapping of Blast-induced Traumatic Brain Injury in Mice Hippocampus

Overview

Journal Sci Data

Specialty Science

Date 2023 Sep 20

PMID 37730716

Authors

Lingxuan Zhang

Qiuyun Yang

Ruixuan Yuan

Manrui Li

Meili Lv

Lin Zhang

Xiaoqi Xie

Weibo Liang

Xiameng Chen

Affiliations

Soon will be listed here.

Abstract

As a significant type of traumatic brain injury (TBI), blast-induced traumatic brain injury (bTBI) frequently results in severe neurological and psychological impairments. Due to its unique mechanistic and clinical features, bTBI presents diagnostic and therapeutic challenges compared to other TBI forms. The hippocampus, an important site for secondary injury of bTBI, serves as a key niche for neural regeneration and repair post-injury, and is closely associated with the neurological outcomes of bTBI patients. Nonetheless, the pathophysiological alterations of hippocampus underpinning bTBI remain enigmatic, and a corresponding transcriptomic dataset for research reference is yet to be established. In this investigation, the single-nucleus RNA sequencing (snRNA-seq) technique was employed to sequence individual hippocampal nuclei of mice from bTBI and sham group. Upon stringent quality control, gene expression data from 17,278 nuclei were obtained, with the dataset's reliability substantiated through various analytical methods. This dataset holds considerable potential for exploring secondary hippocampal injury and neurogenesis mechanisms following bTBI, with important reference value for the identification of specific diagnostic and therapeutic targets for bTBI.

Citing Articles

Short-term neural and glial response to mild traumatic brain injury in the hippocampus.

Dougan C, Roberts B, Crosby A, Karatsoreos I, Peyton S Biophys J. 2024; 123(19):3346-3354.

PMID: 39091025 PMC: 11480756. DOI: 10.1016/j.bpj.2024.07.040.

References

Sun D . Endogenous neurogenic cell response in the mature mammalian brain following traumatic injury. Exp Neurol. 2015; 275 Pt 3:405-410. PMC: 4627904. DOI: 10.1016/j.expneurol.2015.04.017. View

Zeisel A, Munoz-Manchado A, Codeluppi S, Lonnerberg P, La Manno G, Jureus A . Brain structure. Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq. Science. 2015; 347(6226):1138-42. DOI: 10.1126/science.aaa1934. View

Tobin M, Musaraca K, Disouky A, Shetti A, Bheri A, Honer W . Human Hippocampal Neurogenesis Persists in Aged Adults and Alzheimer's Disease Patients. Cell Stem Cell. 2019; 24(6):974-982.e3. PMC: 6608595. DOI: 10.1016/j.stem.2019.05.003. View

Davenport N, Lim K, Armstrong M, Sponheim S . Diffuse and spatially variable white matter disruptions are associated with blast-related mild traumatic brain injury. Neuroimage. 2011; 59(3):2017-24. DOI: 10.1016/j.neuroimage.2011.10.050. View

Ning Y, Zhou Y . Shock tubes and blast injury modeling. Chin J Traumatol. 2016; 18(4):187-93. DOI: 10.1016/j.cjtee.2015.04.005. View

Zhou Y, Su Y, Li S, Kennedy B, Zhang D, Bond A . Molecular landscapes of human hippocampal immature neurons across lifespan. Nature. 2022; 607(7919):527-533. PMC: 9316413. DOI: 10.1038/s41586-022-04912-w. View

Liang J, Cai W, Sun Z . Single-cell sequencing technologies: current and future. J Genet Genomics. 2014; 41(10):513-28. DOI: 10.1016/j.jgg.2014.09.005. View

Nakagawa A, Manley G, Gean A, Ohtani K, Armonda R, Tsukamoto A . Mechanisms of primary blast-induced traumatic brain injury: insights from shock-wave research. J Neurotrauma. 2011; 28(6):1101-19. DOI: 10.1089/neu.2010.1442. View

Gao X, Deng-Bryant Y, Cho W, Carrico K, Hall E, Chen J . Selective death of newborn neurons in hippocampal dentate gyrus following moderate experimental traumatic brain injury. J Neurosci Res. 2008; 86(10):2258-70. PMC: 3757515. DOI: 10.1002/jnr.21677. View

10.

Michinaga S, Koyama Y . Pathophysiological Responses and Roles of Astrocytes in Traumatic Brain Injury. Int J Mol Sci. 2021; 22(12). PMC: 8232783. DOI: 10.3390/ijms22126418. View

11.

Shah P, Stratton J, Stykel M, Abbasi S, Sharma S, Mayr K . Single-Cell Transcriptomics and Fate Mapping of Ependymal Cells Reveals an Absence of Neural Stem Cell Function. Cell. 2018; 173(4):1045-1057.e9. DOI: 10.1016/j.cell.2018.03.063. View

12.

Ratliff W, Mervis R, Citron B, Schwartz B, Rubovitch V, Schreiber S . Effect of mild blast-induced TBI on dendritic architecture of the cortex and hippocampus in the mouse. Sci Rep. 2020; 10(1):2206. PMC: 7010659. DOI: 10.1038/s41598-020-59252-4. View

13.

Bond A, Ming G, Song H . Adult Mammalian Neural Stem Cells and Neurogenesis: Five Decades Later. Cell Stem Cell. 2015; 17(4):385-95. PMC: 4683085. DOI: 10.1016/j.stem.2015.09.003. View

14.

Ayhan F, Kulkarni A, Berto S, Sivaprakasam K, Douglas C, Lega B . Resolving cellular and molecular diversity along the hippocampal anterior-to-posterior axis in humans. Neuron. 2021; 109(13):2091-2105.e6. PMC: 8273123. DOI: 10.1016/j.neuron.2021.05.003. View

15.

Cid E, Marquez-Galera A, Valero M, Gal B, Medeiros D, Navarron C . Sublayer- and cell-type-specific neurodegenerative transcriptional trajectories in hippocampal sclerosis. Cell Rep. 2021; 35(10):109229. DOI: 10.1016/j.celrep.2021.109229. View

16.

Uriarte Huarte O, Kyriakis D, Heurtaux T, Pires-Afonso Y, Grzyb K, Halder R . Single-Cell Transcriptomics and Morphological Analyses Reveal Microglia Heterogeneity Across the Nigrostriatal Pathway. Front Immunol. 2021; 12:639613. PMC: 8039119. DOI: 10.3389/fimmu.2021.639613. View

17.

de Lanerolle N, Kim J, Bandak F . Neuropathology of traumatic brain injury: comparison of penetrating, nonpenetrating direct impact and explosive blast etiologies. Semin Neurol. 2015; 35(1):12-9. DOI: 10.1055/s-0035-1544240. View

18.

Arneson D, Zhang G, Ahn I, Ying Z, Diamante G, Cely I . Systems spatiotemporal dynamics of traumatic brain injury at single-cell resolution reveals humanin as a therapeutic target. Cell Mol Life Sci. 2022; 79(9):480. PMC: 9372016. DOI: 10.1007/s00018-022-04495-9. View

19.

Elder G, Mitsis E, Ahlers S, Cristian A . Blast-induced mild traumatic brain injury. Psychiatr Clin North Am. 2010; 33(4):757-81. DOI: 10.1016/j.psc.2010.08.001. View

20.

Neipert L, Pastorek N, Troyanskaya M, Scheibel R, Petersen N, Levin H . Effect of clinical characteristics on cognitive performance in service members and veterans with histories of blast-related mild traumatic brain injury. Brain Inj. 2014; 28(13-14):1667-74. DOI: 10.3109/02699052.2014.947623. View