Successful 3D Culture and Transplantation of Mouse Isolated Preantral Follicles in Hydrogel of Bioengineered Wharton's Jelly

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2023 Sep 20

PMID 37729236

Authors

Elnaz Zand

Elias Rajablou

Seyedeh Fatemeh Siadat

Bahare Beiki

Vahid Akbarinejad

Christiani Andrade Amorim

Mojtaba Rezazadeh Valojerdi

Leila Alsadat Tahaei

Rouhollah Fathi

Affiliations

Soon will be listed here.

Abstract

Main Objective: Due to Human Wharton's Jelly (HWJ) could be applied in tissue engineering as a bio scaffold, the present study was conducted to investigate the effects of HWJ hydrogel on in vitro culture and auto-transplantation of mouse ovarian follicles.

Materials And Methods: HWJ was isolated from umbilical cord and decellularized with SDS/Tris/EDTA. DNA, Collagen and Glycosaminoglycans (GAGs) were measured. Decellularized Wharton's Jelly (DWJ) was dissolved to make Wharton's Jelly Hydrogel (WJH), and composited with Alginate (ALG) (1.5%) in equal ratio (WJH+ALG). Then, mouse preantral follicles were isolated and encapsulated in 10μL droplets of WJH and randomly considered for both 14 days culture and auto-transplantation.

Results: Collagen, GAGs and DNA evaluations showed majority of WJ cells have been removed and MTT approved no toxicity. Degradation rate and rheological analysis represented optimal hydrogel compatibility. The data from in vitro culture revealed significant antral formation in WJH+ALG (P≤0.05). In transplantation, follicles failed to survive in ALG; however, survived in WJH+ALG to antral stage (P<0.05). VEGF and CD34 had greater expression in WJH+ALG than ALG (P< 0.05).

Conclusion: Wharton's jelly hydrogel and Alginate compound is interesting composite for successful development of mouse preantral follicles in both 3D in vitro culture and transplantation.

Citing Articles

Thiol-yne click crosslink hyaluronic acid/chitosan hydrogel for three-dimensional follicle development.

Khunmanee S, Yoo J, Lee J, Lee J, Park H Mater Today Bio. 2024; 23:100867.

PMID: 38179228 PMC: 10765241. DOI: 10.1016/j.mtbio.2023.100867.

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