The Role of MiR-433-3p in Vascular Calcification in Type 2 Diabetic Patients: Targeting WNT/β-Catenin and RANKL/RANK/OPG Signaling Pathways

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2023 Sep 20

PMID 37728820

Authors

Amira M Elshamy

Yasser Mostafa Hafez

Mohamed A E Safa

Hoda A Ibrahim

Mohamed Khalfallah

Fatma H Rizk

Eman F Eltabaa

Muhammad T Abdel Ghafar

Marwa Mohamed Atef

Affiliations

Soon will be listed here.

Abstract

Background: Vascular calcification (VC) is a major predictor of cardiovascular diseases that represent the principal cause of mortality among type-2 diabetic patients. Accumulating data suggest the vital role of some microRNAs on vascular calcification as an epigenetic regulator. Thus, we assessed herein, the role of serum miR-433-3p in vascular calcification in type-2 diabetic patients.

Methods: Twenty healthy subjects (control group) and forty diabetic patients (20 without VC and 20 with VC) were involved in the study. miR-433-3p gene expression was measured. Runx2, Dickkopf-1 (DKK1), β-catenin, Receptor activator of nuclear factor kappa-B ligand (RANKL), and osteoprotegerin (OPG) levels in serum were assessed by ELISA technique.

Results: Diabetes patients had significantly lower levels of miR-433-3p expression in comparison to the control group, with the lowest levels being found in diabetic patients with VC. Furthermore, Runx2, β-catenin, and RANKL levels were significantly increased with concomitant lower DKK1 and OPG levels detected in the two diabetic groups especially those with VC.

Conclusion: Collectively, the study documented that down-regulation of miR-433-3p may contribute to the development of VC through activating WNT/β-Catenin and RANKL/RANK/OPG signaling pathways.

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