Antimicrobial Activity of Phenyllactic Acid Against Klebsiella Pneumoniae and Its Effect on Cell Wall Membrane and Genomic DNA

Overview

Journal Braz J Microbiol

Specialty Microbiology

Date 2023 Sep 20

PMID 37728681

Authors

Jianyun Yu

Chunli Hong

Longfei Yin

Qingbo Ping

Gaowei Hu

Affiliations

Soon will be listed here.

Abstract

As Klebsiella pneumoniae (KP) has acquired high levels of resistance to multiple antibiotics, it is considered a worldwide pathogen of concern, and substitutes for traditional antibiotics are urgently needed. 3-Phenyllactic acid (PLA) has been reported to have antimicrobial activity against food-borne bacteria. However, there was no experiment evidence for the exact antibacterial effect and mechanism of PLA kills pathogenic KP. In this study, the Oxford cup method indicated that PLA is effective to KP with a minimum inhibitory concentration of 2.5 mg/mL. Furthermore, PLA inhibited the growth and biofilm formation of in a time- and concentration-dependent manner. In vivo, PLA could significantly increase the survival rate of infected mice and reduce the pathological tissue damage. The antibacterial mode of PLA against KP was further explored. Firstly, scanning electron microscopy illustrated the disruption of cellular ultrastructure caused by PLA. Secondly, measurement of leaked alkaline phosphatase demonstrated that PLA disrupted the cell wall integrity of KP and flow cytometry analysis with propidium iodide staining suggested that PLA damaged the cell membrane integrity. Finally, the results of fluorescence spectroscopy and agarose gel electrophoresis demonstrated that PLA bound to genomic DNA and initiated its degradation. The anti-KP mode of action of PLA was attributed to the destruction of the cell wall, membrane, and genomic DNA binding. These findings suggest that PLA has great potential applications as antibiotic substitutes in feed additives against KP infection in animals.

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References

de Souza C, da Silva A, Junior N, Martinez O, Franco O . Peptides as a therapeutic strategy against Klebsiella pneumoniae. Trends Pharmacol Sci. 2022; 43(4):335-348. DOI: 10.1016/j.tips.2021.12.006. View

Tran H, Graham L, Adukwu E . In vitro antifungal activity of Cinnamomum zeylanicum bark and leaf essential oils against Candida albicans and Candida auris. Appl Microbiol Biotechnol. 2020; 104(20):8911-8924. PMC: 7502450. DOI: 10.1007/s00253-020-10829-z. View

Hu Y, Anes J, Devineau S, Fanning S . : Prevalence, Reservoirs, Antimicrobial Resistance, Pathogenicity, and Infection: A Hitherto Unrecognized Zoonotic Bacterium. Foodborne Pathog Dis. 2020; 18(2):63-84. DOI: 10.1089/fpd.2020.2847. View

Song X, Li R, Zhang Q, He S, Wang Y . Antibacterial Effect and Possible Mechanism of Salicylic Acid Microcapsules against and . Int J Environ Res Public Health. 2022; 19(19). PMC: 9566803. DOI: 10.3390/ijerph191912761. View

Sorrentino E, Tremonte P, Succi M, Iorizzo M, Pannella G, Lombardi S . Detection of Antilisterial Activity of 3-Phenyllactic Acid Using as a Model. Front Microbiol. 2018; 9:1373. PMC: 6028618. DOI: 10.3389/fmicb.2018.01373. View

Mobasseri G, Thong K, Teh C . Genomic analysis revealing the resistance mechanisms of extended-spectrum β-lactamase-producing Klebsiella pneumoniae isolated from pig and humans in Malaysia. Int Microbiol. 2021; 24(2):243-250. DOI: 10.1007/s10123-021-00161-5. View

Chatterjee M, DMorris S, Paul V, Warrier S, Vasudevan A, Vanuopadath M . Mechanistic understanding of Phenyllactic acid mediated inhibition of quorum sensing and biofilm development in Pseudomonas aeruginosa. Appl Microbiol Biotechnol. 2017; 101(22):8223-8236. DOI: 10.1007/s00253-017-8546-4. View

Liu F, Sun Z, Wang F, Liu Y, Zhu Y, Du L . Inhibition of biofilm formation and exopolysaccharide synthesis of Enterococcus faecalis by phenyllactic acid. Food Microbiol. 2019; 86:103344. DOI: 10.1016/j.fm.2019.103344. View

Yang F, Deng B, Liao W, Wang P, Chen P, Wei J . High rate of multiresistant from human and animal origin. Infect Drug Resist. 2019; 12:2729-2737. PMC: 6731983. DOI: 10.2147/IDR.S219155. View

10.

Yang Y, Zhang A, Lei C, Wang H, Guan Z, Xu C . Characteristics of Plasmids Coharboring 16S rRNA Methylases, CTX-M, and Virulence Factors in Escherichia coli and Klebsiella pneumoniae Isolates from Chickens in China. Foodborne Pathog Dis. 2015; 12(11):873-80. DOI: 10.1089/fpd.2015.2025. View

11.

Durdu B, Hakyemez I, Bolukcu S, Okay G, Gultepe B, Aslan T . Mortality markers in nosocomial bloodstream infection. Springerplus. 2016; 5(1):1892. PMC: 5084144. DOI: 10.1186/s40064-016-3580-8. View

12.

Wang J, Yoo J, Lee J, Jang H, Kim H, Shin S . Effects of phenyllactic acid on growth performance, nutrient digestibility, microbial shedding, and blood profile in pigs. J Anim Sci. 2009; 87(10):3235-43. DOI: 10.2527/jas.2008-1555. View

13.

Bernardini A, Cuesta T, Tomas A, Bengoechea J, Martinez J, Sanchez M . The intrinsic resistome of Klebsiella pneumoniae. Int J Antimicrob Agents. 2018; 53(1):29-33. DOI: 10.1016/j.ijantimicag.2018.09.012. View

14.

Ning Y, Yan A, Yang K, Wang Z, Li X, Jia Y . Antibacterial activity of phenyllactic acid against Listeria monocytogenes and Escherichia coli by dual mechanisms. Food Chem. 2017; 228:533-540. DOI: 10.1016/j.foodchem.2017.01.112. View

15.

Rasheed J, Anderson G, Yigit H, Queenan A, Domenech-Sanchez A, Swenson J . Characterization of the extended-spectrum beta-lactamase reference strain, Klebsiella pneumoniae K6 (ATCC 700603), which produces the novel enzyme SHV-18. Antimicrob Agents Chemother. 2000; 44(9):2382-8. PMC: 90073. DOI: 10.1128/AAC.44.9.2382-2388.2000. View

16.

Zhang J, Cui X, Zhang M, Bai B, Yang Y, Fan S . The antibacterial mechanism of perilla rosmarinic acid. Biotechnol Appl Biochem. 2021; 69(4):1757-1764. DOI: 10.1002/bab.2248. View

17.

Qian W, Sun Z, Wang T, Yang M, Liu M, Zhang J . Antimicrobial activity of eugenol against carbapenem-resistant Klebsiella pneumoniae and its effect on biofilms. Microb Pathog. 2019; 139:103924. DOI: 10.1016/j.micpath.2019.103924. View

18.

Brudzynski K, Abubaker K, Miotto D . Unraveling a mechanism of honey antibacterial action: polyphenol/H₂O₂-induced oxidative effect on bacterial cell growth and on DNA degradation. Food Chem. 2015; 133(2):329-36. DOI: 10.1016/j.foodchem.2012.01.035. View

19.

Zou L, Wang H, Zeng B, Zhang A, Li J, Li X . Phenotypic and genotypic characterization of β-lactam resistance in Klebsiella pneumoniae isolated from swine. Vet Microbiol. 2010; 149(1-2):139-46. DOI: 10.1016/j.vetmic.2010.09.030. View

20.

Wang F, Wu H, Jin P, Sun Z, Liu F, Du L . Antimicrobial Activity of Phenyllactic Acid Against Enterococcus faecalis and Its Effect on Cell Membrane. Foodborne Pathog Dis. 2018; 15(10):645-652. DOI: 10.1089/fpd.2018.2470. View