Prevalence of Mutations Associated with Macrolide and Fluoroquinolone Resistance in Neisseria Gonorrhoeae with Allplex NG-DR Assay (Seegene) in a Tertiary Hospital from Madrid, Spain

Overview

Journal Rev Esp Quimioter

Specialty Pharmacology

Date 2023 Sep 18

PMID 37721708

Authors

A Maldonado-Barrueco

C Sanz-Gonzalez

I Falces-Romero

P Garcia-Clemente

J Cacho-Calvo

I Quiles-Melero

Affiliations

Soon will be listed here.

Abstract

Objective: The prevalence of drug-resistant Neisseria gonorrhoeae (NG) infections is increasing. Studies report the prevalence of NG strains presenting A2059G/C2611T (rRNA23S) and S91F (parC) mutations conferring resistance to azithromycin and ciprofloxacin.

Methods: We conducted a prospective cohort study evaluating first void-urine urines, rectal, and oropharyngeal swabs collected from a cohort of patients in a tertiary hospital in Madrid between October 2022 and January 2023. Samples were screened by Allplex 7-STI Essential Assay (Seegene). Drug resistances were performed by Allplex NG-DR Assay (Seegene).

Results: A total of 1,415 patients were included, of which 112 had a positive sample for NG infection. One patient had a C2611T mutation (0.9%) and neither patient showed A2059G mutation. We found 67 (59.8%) S91F-positive patients. Forty-four patients (39.3%) not had any mutations.

Conclusions: We report a low-prevalence of mutations A2059G/C2611T to macrolides and a high-prevalence to S91F in NG infections. Molecular methods for the detection of NG resistance could be useful in direct non-culturable samples.

Citing Articles

AllplexTM NG-DR (Seegene) utility for switching oral regimens with fluoroquinolones in gonococcal arthritis.

Maldonado-Barrueco A, Sanz-Gonzalez C, Grandioso-Vas D, Falces-Romero I, Garcia-Rodriguez J, Quiles-Melero I Rev Esp Quimioter. 2024; 37(5):425-426.

PMID: 38946479 PMC: 11462318. DOI: 10.37201/req/030.2024.

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