Two-dimensional Liquid Chromatography and Ion Mobility-mass Spectrometry for the Multicomponent Characterization of Different Parts of the Medicinal Plant

Overview

Journal Front Chem

Specialty Chemistry

Date 2023 Sep 18

PMID 37720716

Authors

Wei Zheng

Gang Li

Guang Yang

Pengxin Lu

Qi Li

Mengmeng Zhang

Ming Yuan

Xiaojuan Chen

Chenchen Wang

Baolin Guo

Baiping Ma

Affiliations

Soon will be listed here.

Abstract

Herba Gynostemma (Jiaogulan) is an herbaceous plant of the genus in the family Cucurbitaceae. has lipid-lowering activity, thus, it is used as a medicinal material. However, its medicinal using parts have been recorded as whole plants or aerial parts in different provincial quality standards; therefore, it is necessary to conduct a comprehensive compositional analysis of the different parts of (rhizomes, stems, and leaves) used in traditional medicine. In this study, offline two-dimensional liquid chromatography-ion mobility-quadrupole time-of-flight mass spectrometry (2D-LC/IM-QTOF-MS) was used to analyze the different parts of obtained from Shaanxi province, China. By combining the retention times, mass fragments, collision cross-section values, reference standards, and information concerning literature compounds, 396 components were identified from the three parts of the plant, including 94 groups of isomers, and 217 components were identified or tentatively identified as new compounds. In the rhizomes, leaves, and stems, 240, 220, and 168 compounds, respectively, were identified. Differential analysis of the compounds in the rhizomes and aerial parts was also carried out, and 36 differential components were identified, of which 32 had higher contents in the rhizomes. Therefore, these findings indicate that the number of chemical components and the content of major differential components are higher in the rhizomes than the leaves and stems of from the Maobaling Planting Base in Pingli county, Shaanxi province. Thus, the rhizomes of are also an important part for medicinal use. These results will contribute to the establishment of quality control methods for .

References

Wang M, Xu X, Wang H, Wang H, Liu M, Hu W . A multi-dimensional liquid chromatography/high-resolution mass spectrometry approach combined with computational data processing for the comprehensive characterization of the multicomponents from Cuscuta chinensis. J Chromatogr A. 2022; 1675:463162. DOI: 10.1016/j.chroma.2022.463162. View

Wang X, Li D, Guo X, Zhang Q, Liao X, Cao Z . ComMSDB-An Automatable Strategy to Identify Compounds from MS Data Sets (Identification of Gypenosides as an Example). J Agric Food Chem. 2020; 68(41):11368-11388. DOI: 10.1021/acs.jafc.0c03693. View

Huang G, Yasir M, Zheng Y, Khan I . Prebiotic properties of jiaogulan in the context of gut microbiome. Food Sci Nutr. 2022; 10(3):731-739. PMC: 8907712. DOI: 10.1002/fsn3.2701. View

Song X, Canellas E, Dreolin N, Goshawk J, Nerin C . A Collision Cross Section Database for Extractables and Leachables from Food Contact Materials. J Agric Food Chem. 2022; 70(14):4457-4466. PMC: 9011387. DOI: 10.1021/acs.jafc.2c00724. View

Schroeder M, Meyer S, Heyman H, Barsch A, Sumner L . Generation of a Collision Cross Section Library for Multi-Dimensional Plant Metabolomics Using UHPLC-Trapped Ion Mobility-MS/MS. Metabolites. 2019; 10(1). PMC: 7023306. DOI: 10.3390/metabo10010013. View

Shu X, Chen R, Yang M, Xu J, Gao R, Hu Y . Gynostemma pentaphyllum and Gypenoside-IV Ameliorate Metabolic Disorder and Gut Microbiota in Diet-Induced-Obese Mice. Plant Foods Hum Nutr. 2022; 77(3):367-372. DOI: 10.1007/s11130-022-00982-3. View

Li G, Lu P, Liang H, Zheng W, Chen X, Zhang J . An effective and high-throughput sample preparation method involving demalonylation followed by an ultrahigh-performance liquid chromatography-charged aerosol detector for analyzing gypenoside XLIX and gypenoside A in Gynostemma longipes. J Pharm Biomed Anal. 2023; 230:115393. DOI: 10.1016/j.jpba.2023.115393. View

Pham H, Quy Ha T, Cho H, Lee B, An J, Tran V . Insulin Mimetic Activity of 3,4- Seco and Hexanordammarane Triterpenoids Isolated from Gynostemma longipes. J Nat Prod. 2018; 81(11):2470-2482. DOI: 10.1021/acs.jnatprod.8b00524. View

Qiu S, Yang W, Shi X, Yao C, Yang M, Liu X . A green protocol for efficient discovery of novel natural compounds: characterization of new ginsenosides from the stems and leaves of Panax ginseng as a case study. Anal Chim Acta. 2015; 893:65-76. DOI: 10.1016/j.aca.2015.08.048. View

10.

Chen C, Chen H, Zhang Y, Thomas H, Frank M, He Y . TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data. Mol Plant. 2020; 13(8):1194-1202. DOI: 10.1016/j.molp.2020.06.009. View

11.

Vasilopoulou C, Sulek K, Brunner A, Meitei N, Schweiger-Hufnagel U, Meyer S . Trapped ion mobility spectrometry and PASEF enable in-depth lipidomics from minimal sample amounts. Nat Commun. 2020; 11(1):331. PMC: 6965134. DOI: 10.1038/s41467-019-14044-x. View

12.

Yin M, Zhang J, Wang L, Li F, Li Z, Xiang W . Ten New Dammarane-Type Saponins with Hypolipidemia Activity from a Functional Herbal Tea-. Molecules. 2020; 25(16). PMC: 7464191. DOI: 10.3390/molecules25163737. View

13.

Connolly J, Munoz-Muriedas J, Lapthorn C, Higton D, Vissers J, Webb A . Investigation into Small Molecule Isomeric Glucuronide Metabolite Differentiation Using and Experimental Collision Cross-Section Values. J Am Soc Mass Spectrom. 2021; 32(8):1976-1986. DOI: 10.1021/jasms.0c00427. View

14.

Teng F, Li X, Li M, Fan D, Zhu J, Gao H . [Components and lipid-lowering effect of total saponins from underground part of Gynostemma pentaphyllum]. Zhongguo Zhong Yao Za Zhi. 2022; 47(18):5022-5031. DOI: 10.19540/j.cnki.cjcmm.20220613.701. View

15.

Zhang M, Zheng W, Zhang J, Gao L, Liu X, Luo D . [Qualitative analysis of Gynostemma longipes for medicinal usage]. Zhongguo Zhong Yao Za Zhi. 2021; 46(4):951-965. DOI: 10.19540/j.cnki.cjcmm.20200623.204. View

16.

Broeckling C, Yao L, Isaac G, Gioioso M, Ianchis V, Vissers J . Application of Predicted Collisional Cross Section to Metabolome Databases to Probabilistically Describe the Current and Future Ion Mobility Mass Spectrometry. J Am Soc Mass Spectrom. 2021; 32(3):661-669. DOI: 10.1021/jasms.0c00375. View

17.

Zhou Z, Luo M, Chen X, Yin Y, Xiong X, Wang R . Ion mobility collision cross-section atlas for known and unknown metabolite annotation in untargeted metabolomics. Nat Commun. 2020; 11(1):4334. PMC: 7455731. DOI: 10.1038/s41467-020-18171-8. View

18.

Zu M, Piao X, Gao J, Xing S, Liu L . Monomer gypenoside LI from Gynostemma pentaphyllum inhibits cell proliferation and upregulates expression of miR-128-3p in melanoma cells. J Biochem Mol Toxicol. 2020; 34(5):e22460. DOI: 10.1002/jbt.22460. View

19.

Zhou Z, Tu J, Zhu Z . Advancing the large-scale CCS database for metabolomics and lipidomics at the machine-learning era. Curr Opin Chem Biol. 2017; 42:34-41. DOI: 10.1016/j.cbpa.2017.10.033. View

20.

Zhang C, Zuo T, Wang X, Wang H, Hu Y, Li Z . Integration of Data-Dependent Acquisition (DDA) and Data-Independent High-Definition MS (HDMS) for the Comprehensive Profiling and Characterization of Multicomponents from by UHPLC/IM-QTOF-MS. Molecules. 2019; 24(15). PMC: 6695638. DOI: 10.3390/molecules24152708. View