Multiexposure Laser Speckle Contrast Analysis System Calibration Limited by Perfusion-dependent Scattering on the Skin

Overview

Journal J Biomed Opt

Specialties Biomedical Engineering
Ophthalmology

Date 2023 Sep 18

PMID 37720190

Authors

Tamas Smausz

Bence Kondasz

Affiliations

Soon will be listed here.

Abstract

Significance: Application of multiexposure speckle contrast imaging (MESI) methods for perfusion measurements can correct for the contribution of static scattering of the skin, at the expense of reduced temporal resolution as compared to classical single-exposure methods. Persistence of tissue scattering properties during the measurements could allow for an initial calibration and enhancement of the temporal resolution of the measurements.

Aim: We aim to study the influence of the perfusion on the light scattering of the forearm skin and to use the obtained data for the enhancement of the temporal resolution.

Approach: A wide range of skin perfusion states was induced while monitoring the changes in the dynamic range of the exposure-dependent contrast. Different measurement and evaluation methods were tested based on an initial MESI calibration followed by image recording with reduced number of exposure time values.

Results: The changes in the skin perfusion can alter not only the contribution of the static scattering to the speckle images but also the short-exposure time contrast limit.

Conclusions: The perfusion-dependent scattering of the skin can invalidate the precalibrations (e.g., calibration) characterizing the combination of the given tissue and the measurement system.

References

Wilkinson J, Leggett S, Marjanovic E, Moore T, Allen J, Anderson M . A Multicenter Study of the Validity and Reliability of Responses to Hand Cold Challenge as Measured by Laser Speckle Contrast Imaging and Thermography: Outcome Measures for Systemic Sclerosis-Related Raynaud's Phenomenon. Arthritis Rheumatol. 2018; 70(6):903-911. PMC: 6001804. DOI: 10.1002/art.40457. View

Farnebo S, Thorfinn J, Henricson J, Tesselaar E . Hyperaemic changes in forearm skin perfusion and RBC concentration after increasing occlusion times. Microvasc Res. 2010; 80(3):412-6. DOI: 10.1016/j.mvr.2010.07.008. View

Colin E, Plyer A, Golzio M, Meyer N, Favre G, Orlik X . Imaging of the skin microvascularization using spatially depolarized dynamic speckle. J Biomed Opt. 2022; 27(4). PMC: 9043838. DOI: 10.1117/1.JBO.27.4.046003. View

Pauling J, Shipley J, Raper S, Watson M, Ward S, Harris N . Comparison of infrared thermography and laser speckle contrast imaging for the dynamic assessment of digital microvascular function. Microvasc Res. 2011; 83(2):162-7. DOI: 10.1016/j.mvr.2011.06.012. View

Fox R, WYATT H . Cold-induced vasodilatation in various areas of the body surface of man. J Physiol. 1962; 162:289-97. PMC: 1359658. DOI: 10.1113/jphysiol.1962.sp006933. View

Holmer A, Tetschke F, Marotz J, Malberg H, Markgraf W, Thiele C . Oxygenation and perfusion monitoring with a hyperspectral camera system for chemical based tissue analysis of skin and organs. Physiol Meas. 2016; 37(11):2064-2078. DOI: 10.1088/0967-3334/37/11/2064. View

Andersen H, Poulsen J, Uchida Y, Nikbakht A, Arendt-Nielsen L, Gazerani P . Cold and L-menthol-induced sensitization in healthy volunteers--a cold hypersensitivity analogue to the heat/capsaicin model. Pain. 2015; 156(5):880-889. DOI: 10.1097/j.pain.0000000000000123. View

Boas D, Dunn A . Laser speckle contrast imaging in biomedical optics. J Biomed Opt. 2010; 15(1):011109. PMC: 2816990. DOI: 10.1117/1.3285504. View

Khaksari K, Kirkpatrick S . Combined effects of scattering and absorption on laser speckle contrast imaging. J Biomed Opt. 2016; 21(7):76002. DOI: 10.1117/1.JBO.21.7.076002. View

10.

Stewart C, Frank R, Forrester K, Tulip J, Lindsay R, Bray R . A comparison of two laser-based methods for determination of burn scar perfusion: laser Doppler versus laser speckle imaging. Burns. 2005; 31(6):744-52. DOI: 10.1016/j.burns.2005.04.004. View

11.

Mirdell R, Iredahl F, Sjoberg F, Farnebo S, Tesselaar E . Microvascular blood flow in scalds in children and its relation to duration of wound healing: A study using laser speckle contrast imaging. Burns. 2016; 42(3):648-54. DOI: 10.1016/j.burns.2015.12.005. View

12.

Casavola C, Paunescu L, Fantini S, Gratton E . Blood flow and oxygen consumption with near-infrared spectroscopy and venous occlusion: spatial maps and the effect of time and pressure of inflation. J Biomed Opt. 2000; 5(3):269-76. DOI: 10.1117/1.429995. View

13.

Chen X, Lin W, Wang C, Chen S, Sheng J, Zeng B . real-time imaging of cutaneous hemoglobin concentration, oxygen saturation, scattering properties, melanin content, and epidermal thickness with visible spatially modulated light. Biomed Opt Express. 2018; 8(12):5468-5482. PMC: 5745096. DOI: 10.1364/BOE.8.005468. View

14.

Dupont J, Orlik X, Ghabbach A, Zerrad M, Soriano G, Amra C . Polarization analysis of speckle field below its transverse correlation width : application to surface and bulk scattering. Opt Express. 2014; 22(20):24133-41. DOI: 10.1364/OE.22.024133. View

15.

van Manen L, Birkhoff W, Eggermont J, Hoveling R, Nicklin P, Burggraaf J . Detection of cutaneous oxygen saturation using a novel snapshot hyperspectral camera: a feasibility study. Quant Imaging Med Surg. 2021; 11(9):3966-3977. PMC: 8339658. DOI: 10.21037/qims-21-46. View

16.

Dragojevic T, Bronzi D, Varma H, Valdes C, Castellvi C, Villa F . High-speed multi-exposure laser speckle contrast imaging with a single-photon counting camera. Biomed Opt Express. 2015; 6(8):2865-76. PMC: 4541515. DOI: 10.1364/BOE.6.002865. View

17.

Basak K, Dey G, Mahadevappa M, Mandal M, Sheet D, Dutta P . Learning of speckle statistics for in vivo and noninvasive characterization of cutaneous wound regions using laser speckle contrast imaging. Microvasc Res. 2016; 107:6-16. DOI: 10.1016/j.mvr.2016.04.008. View

18.

Parthasarathy A, Tom W, Gopal A, Zhang X, Dunn A . Robust flow measurement with multi-exposure speckle imaging. Opt Express. 2008; 16(3):1975-89. DOI: 10.1364/oe.16.001975. View

19.

Ruth B, Schmand J, Abendroth D . Noncontact determination of skin blood flow using the laser speckle method: application to patients with peripheral arterial occlusive disease (PAOD) and to type-I diabetics. Lasers Surg Med. 1993; 13(2):179-88. DOI: 10.1002/lsm.1900130205. View

20.

Zakharov P, Volker A, Buck A, Weber B, Scheffold F . Quantitative modeling of laser speckle imaging. Opt Lett. 2006; 31(23):3465-7. DOI: 10.1364/ol.31.003465. View