Tissue Distribution of MtDNA Copy Number And Expression Pattern of An MtDNA-Related Gene in Three Teleost Fish Species

Overview

Journal Integr Org Biol

Publisher Oxford University Press

Specialty Biology

Date 2023 Sep 14

PMID 37705694

Authors

B Li

H Wang

C Jiang

X Zeng

T Zhang

S Liu

Z Zhuang

Affiliations

Soon will be listed here.

Abstract

Teleosts are the most speciose vertebrates and have diverse swimming performance. Based on swimming duration and speed, teleosts are broadly divided into sustained, prolonged, and burst swimming fish. Teleosts with different swimming performance have different energy requirements. In addition, energy requirement also varies among different tissues. As mitochondrial DNA (mtDNA) copy number is correlated with ATP production, we speculated that mtDNA copy number varies among fish with different swimming performance, as well as among different tissues. In other species, mtDNA copy number is regulated by (mitochondrial transcription factor A) through mtDNA compaction and mito-genome replication initiation. In order to clarify the tissue distribution of mtDNA copy number and expression pattern of in teleosts with disparate swimming performance, we selected representative fish with sustained swimming (), prolonged swimming (), and burst swimming (). We measured mtDNA copy number and gene expression in 10 tissues of these three fish. The results showed the mtDNA content pattern of various tissues was broadly consistent among three fish, and high-energy demanding tissues contain higher mtDNA copy number. Slow-twitch muscles with higher oxidative metabolism possess a greater content of mtDNA than fast-twitch muscles. In addition, relatively higher mtDNA content in fast-twitch muscle of compared to the other two fish could be an adaptation to their frequent burst swimming demands. And the higher mtDNA copy number in heart of could meet their oxygen transport demands of long-distance swimming. However, expression was not significantly correlated with mtDNA copy number in these teleosts, suggesting may be not the only factor regulating mtDNA content among various tissues. This study can lay a foundation for studying the role of mtDNA in the adaptive evolution of various swimming ability in teleost fish.

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