Functional Knockout of Long Non-coding RNAs with Genome Editing

Overview

Journal Front Genet

Date 2023 Sep 14

PMID 37705609

Authors

Qing Rex Lyu

Shikuan Zhang

Zhe Zhang

Zhiyu Tang

Affiliations

Soon will be listed here.

Abstract

An effective loss-of-function study is necessary to investigate the biological function of long non-coding RNA (lncRNA). Various approaches are available, including RNA silencing, antisense oligos, and CRISPR-based genome editing. CRISPR-based genome editing is the most widely used for inactivating lncRNA function at the genomic level. Knocking out the lncRNA function can be achieved by removing the promoter and the first exon (PE1), introducing pre-termination poly(A) signals, or deleting the entire locus, unlike frameshift strategies used for messenger RNA (mRNA). However, the intricate genomic interplay between lncRNA and neighbor genes makes it challenging to interpret lncRNA function accurately. This article discusses the advantages and disadvantages of each lncRNA knockout method and envisions the potential future directions to facilitate lncRNA functional study.

Citing Articles

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