MiR-638 Suppresses Cervical Cancer Progression by Inhibiting Under the Treatment of Tetrandrine

Overview

Journal Histol Histopathol

Specialties Anatomy & Histology
Pathology

Date 2023 Sep 13

PMID 37702425

Authors

Min Wang

Kai Liu

Zhongming Zhou

Huizhuan Geng

Affiliations

Soon will be listed here.

Abstract

Background: The interaction of microRNA with Chinese herbal medicines is a promising therapeutic approach for prevention of cervical cancer.

Methods: Western blotting or qRT-PCR were carried out to identify the expression of and miR-638. A tetrandrine (TET) cell model was used to explore the effects of miR-638 and its target gene using CCK-8, transwell, wound healing, and western blot assays. Furthermore, luciferase activity assay was conducted to measure the interaction among TET, and miR-638.

Results: Under TET treatment, Hela and SiHa cells exhibited repressed cell viability, migration, invasion, and epithelial-mesenchymal transition (EMT), and these effects were further enhanced by high expression of miR-638. In contrast, expression was low in TET-treated cells and had an opposite effect to that of miR-638.

Conclusion: We highlighted that miR-638 suppresses cervical cancer progression by inhibiting under tetrandrine treatment.

References

Agarwal V, Bell G, Nam J, Bartel D . Predicting effective microRNA target sites in mammalian mRNAs. Elife. 2015; 4. PMC: 4532895. DOI: 10.7554/eLife.05005. View

N B, K R C . Tetrandrine and cancer - An overview on the molecular approach. Biomed Pharmacother. 2017; 97:624-632. DOI: 10.1016/j.biopha.2017.10.116. View

Burd E . Human papillomavirus and cervical cancer. Clin Microbiol Rev. 2003; 16(1):1-17. PMC: 145302. DOI: 10.1128/CMR.16.1.1-17.2003. View

Chen Y . Potential role of tetrandrine in cancer therapy. Acta Pharmacol Sin. 2002; 23(12):1102-6. View

Chen Z, Zhao L, Zhao F, Yang G, Wang J . Tetrandrine suppresses lung cancer growth and induces apoptosis, potentially via the VEGF/HIF-1α/ICAM-1 signaling pathway. Oncol Lett. 2018; 15(5):7433-7437. PMC: 5962877. DOI: 10.3892/ol.2018.8190. View

Chow L, Cheng K, Leong F, Cheung C, Shiao L, Leung Y . Enhancing tetrandrine cytotoxicity in human lung carcinoma A549 cells by suppressing mitochondrial ATP production. Naunyn Schmiedebergs Arch Pharmacol. 2018; 392(4):427-436. DOI: 10.1007/s00210-018-01601-2. View

Chu Y, Zhu Y, Zhang Y, Liu X, Guo Y, Chang L . Tetrandrine attenuates intestinal epithelial barrier defects caused by colitis through promoting the expression of Occludin via the AhR-miR-429 pathway. FASEB J. 2021; 35(5):e21502. DOI: 10.1096/fj.202002086RR. View

Cui X, Xiao D, Wang X . Inhibition of laryngeal cancer stem cells by tetrandrine. Anticancer Drugs. 2019; 30(9):886-891. DOI: 10.1097/CAD.0000000000000803. View

den Boon J, Pyeon D, Wang S, Horswill M, Schiffman M, Sherman M . Molecular transitions from papillomavirus infection to cervical precancer and cancer: Role of stromal estrogen receptor signaling. Proc Natl Acad Sci U S A. 2015; 112(25):E3255-64. PMC: 4485108. DOI: 10.1073/pnas.1509322112. View

10.

Fang J, Zhang H, Jin S . Epigenetics and cervical cancer: from pathogenesis to therapy. Tumour Biol. 2014; 35(6):5083-93. DOI: 10.1007/s13277-014-1737-z. View

11.

Khan T, Khan K, Sadeghpour A, Reynolds H, Perilla Y, McDonald M . Mutations in NCAPG2 Cause a Severe Neurodevelopmental Syndrome that Expands the Phenotypic Spectrum of Condensinopathies. Am J Hum Genet. 2019; 104(1):94-111. PMC: 6323578. DOI: 10.1016/j.ajhg.2018.11.017. View

12.

Kuroda H, Nakazawa S, Katagiri K, SHIRATORI O, Kozuka M . Antitumor effect of bisbenzylisoquinoline alkaloids. Chem Pharm Bull (Tokyo). 1976; 24(10):2413-20. DOI: 10.1248/cpb.24.2413. View

13.

Kwan C, Achike F . Tetrandrine and related bis-benzylisoquinoline alkaloids from medicinal herbs: cardiovascular effects and mechanisms of action. Acta Pharmacol Sin. 2002; 23(12):1057-68. View

14.

Laengsri V, Kerdpin U, Plabplueng C, Treeratanapiboon L, Nuchnoi P . Cervical Cancer Markers: Epigenetics and microRNAs. Lab Med. 2018; 49(2):97-111. DOI: 10.1093/labmed/lmx080. View

15.

Liao D, Zhang W, Gupta P, Lei Z, Wang J, Cai C . Tetrandrine Interaction with ABCB1 Reverses Multidrug Resistance in Cancer Cells Through Competition with Anti-Cancer Drugs Followed by Downregulation of ABCB1 Expression. Molecules. 2019; 24(23). PMC: 6930469. DOI: 10.3390/molecules24234383. View

16.

Liu Z, Hirano T, Tanaka S, Onda K, Oka K . Persistent reversal of P-glycoprotein-mediated daunorubicin resistance by tetrandrine in multidrug-resistant human T lymphoblastoid leukemia MOLT-4 cells. J Pharm Pharmacol. 2004; 55(11):1531-7. DOI: 10.1211/0022357022115. View

17.

Meng F, Zhang S, Song R, Liu Y, Wang J, Liang Y . NCAPG2 overexpression promotes hepatocellular carcinoma proliferation and metastasis through activating the STAT3 and NF-κB/miR-188-3p pathways. EBioMedicine. 2019; 44:237-249. PMC: 6606561. DOI: 10.1016/j.ebiom.2019.05.053. View

18.

Moore D . Cervical cancer. Obstet Gynecol. 2006; 107(5):1152-61. DOI: 10.1097/01.AOG.0000215986.48590.79. View

19.

Mori M, Ludwig R, Garcia-Martin R, Brandao B, Kahn C . Extracellular miRNAs: From Biomarkers to Mediators of Physiology and Disease. Cell Metab. 2019; 30(4):656-673. PMC: 6774861. DOI: 10.1016/j.cmet.2019.07.011. View

20.

Olusola P, Banerjee H, Philley J, Dasgupta S . Human Papilloma Virus-Associated Cervical Cancer and Health Disparities. Cells. 2019; 8(6). PMC: 6628030. DOI: 10.3390/cells8060622. View