Bio-conjugated Nanoarchitectonics with Dual-labeled Nanoparticles for a Colorimetric and Fluorescent Dual-mode Serological Lateral Flow Immunoassay Sensor in Detection of SARS-CoV-2 in Clinical Samples

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2023 Sep 13

PMID 37701275

Authors

Sang Ki Kim

Jong Uk Lee

Myeong Jin Jeon

Soo-Kyung Kim

Sang-Hyun Hwang

Min Eui Hong

Sang Jun Sim

Affiliations

Soon will be listed here.

Abstract

Serological detection of antibodies for diagnosing infectious diseases has advantages in facile diagnostic procedures, thereby contributing to controlling the spread of the pathogen, such as in the recent SARS-CoV-2 pandemic. Lateral flow immunoassay (LFIA) is a representative serological antibody detection method suitable for on-site applications but suffers from low clinical accuracy. To achieve a simple and rapid serological screening as well as the sensitive quantification of antibodies against SARS-CoV-2, a colorimetric and fluorescent dual-mode serological LFIA sensor incorporating metal-enhanced fluorescence (MEF) was developed. For the strong fluorescence signal amplification, fluorophore Cy3 was immobilized onto gold nanoparticles (AuNPs) with size-controllable spacer polyethyleneglycol (PEG) to maintain an optimal distance to induce MEF. The sensor detects the target IgG with a concentration as low as 1 ng mL within 8 minutes. The employment of the MEF into the dual-mode serological LFIA sensor shows a 1000-fold sensitivity improvement compared with that of colorimetric LFIAs. The proposed serological LFIA sensor was tested with 73 clinical samples, showing sensitivity, specificity, and accuracy of 95%, 100%, and 97%, respectively. In conclusion, the dual-mode serological LFIA has great potential for application in diagnosis and an epidemiological survey of vaccine efficacy and immunity status of individuals.

Citing Articles

Materials Nanoarchitectonics for Advanced Devices.

Ariga K Materials (Basel). 2024; 17(23).

PMID: 39685353 PMC: 11643071. DOI: 10.3390/ma17235918.

Liquid-Liquid and Liquid-Solid Interfacial Nanoarchitectonics.

Ariga K Molecules. 2024; 29(13).

PMID: 38999120 PMC: 11243083. DOI: 10.3390/molecules29133168.

Recent Advances in Lateral Flow Assays for Viral Protein Detection with Nanomaterial-Based Optical Sensors.

Kim M, Haizan I, Ahn M, Park D, Choi J Biosensors (Basel). 2024; 14(4).

PMID: 38667190 PMC: 11048458. DOI: 10.3390/bios14040197.

2D Materials Nanoarchitectonics for 3D Structures/Functions.

Ariga K Materials (Basel). 2024; 17(4).

PMID: 38399187 PMC: 10890396. DOI: 10.3390/ma17040936.

Confined Space Nanoarchitectonics for Dynamic Functions and Molecular Machines.

Ariga K Micromachines (Basel). 2024; 15(2).

PMID: 38399010 PMC: 10892885. DOI: 10.3390/mi15020282.

References

Zhang L, Huang Y, Wang J, Rong Y, Lai W, Zhang J . Hierarchical Flowerlike Gold Nanoparticles Labeled Immunochromatography Test Strip for Highly Sensitive Detection of Escherichia coli O157:H7. Langmuir. 2015; 31(19):5537-44. DOI: 10.1021/acs.langmuir.5b00592. View

Jeong Y, Kook Y, Lee K, Koh W . Metal enhanced fluorescence (MEF) for biosensors: General approaches and a review of recent developments. Biosens Bioelectron. 2018; 111:102-116. DOI: 10.1016/j.bios.2018.04.007. View

Dutta D, Naiyer S, Mansuri S, Soni N, Singh V, Bhat K . COVID-19 Diagnosis: A Comprehensive Review of the RT-qPCR Method for Detection of SARS-CoV-2. Diagnostics (Basel). 2022; 12(6). PMC: 9221722. DOI: 10.3390/diagnostics12061503. View

Qu J, Leirs K, Maes W, Imbrechts M, Callewaert N, Lagrou K . Innovative FO-SPR Label-free Strategy for Detecting Anti-RBD Antibodies in COVID-19 Patient Serum and Whole Blood. ACS Sens. 2022; 7(2):477-487. DOI: 10.1021/acssensors.1c02215. View

Streiner D, Cairney J . What's under the ROC? An introduction to receiver operating characteristics curves. Can J Psychiatry. 2007; 52(2):121-8. DOI: 10.1177/070674370705200210. View

Fluss R, Faraggi D, Reiser B . Estimation of the Youden Index and its associated cutoff point. Biom J. 2005; 47(4):458-72. DOI: 10.1002/bimj.200410135. View

Tang D, Sauceda J, Lin Z, Ott S, Basova E, Goryacheva I . Magnetic nanogold microspheres-based lateral-flow immunodipstick for rapid detection of aflatoxin B2 in food. Biosens Bioelectron. 2009; 25(2):514-8. DOI: 10.1016/j.bios.2009.07.030. View

Li R, Meng C, Wen Y, Fu W, He P . Fluorometric lateral flow immunoassay for simultaneous determination of three mycotoxins (aflatoxin B, zearalenone and deoxynivalenol) using quantum dot microbeads. Mikrochim Acta. 2019; 186(12):748. DOI: 10.1007/s00604-019-3879-6. View

West C, Montori V, Sampathkumar P . COVID-19 Testing: The Threat of False-Negative Results. Mayo Clin Proc. 2020; 95(6):1127-1129. PMC: 7151274. DOI: 10.1016/j.mayocp.2020.04.004. View

10.

Lyashchenko K, Singh M, Colangeli R, Gennaro M . A multi-antigen print immunoassay for the development of serological diagnosis of infectious diseases. J Immunol Methods. 2000; 242(1-2):91-100. DOI: 10.1016/s0022-1759(00)00241-6. View

11.

Hwang W, Truong P, Sim S . Size-dependent plasmonic responses of single gold nanoparticles for analysis of biorecognition. Anal Biochem. 2011; 421(1):213-8. DOI: 10.1016/j.ab.2011.11.001. View

12.

Sekimukai H, Iwata-Yoshikawa N, Fukushi S, Tani H, Kataoka M, Suzuki T . Gold nanoparticle-adjuvanted S protein induces a strong antigen-specific IgG response against severe acute respiratory syndrome-related coronavirus infection, but fails to induce protective antibodies and limit eosinophilic infiltration in lungs. Microbiol Immunol. 2019; 64(1):33-51. PMC: 7168429. DOI: 10.1111/1348-0421.12754. View

13.

Acter T, Uddin N, Das J, Akhter A, Choudhury T, Kim S . Evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as coronavirus disease 2019 (COVID-19) pandemic: A global health emergency. Sci Total Environ. 2020; 730:138996. PMC: 7190497. DOI: 10.1016/j.scitotenv.2020.138996. View

14.

Choi J, Lim J, Shin M, Paek S, Choi J . CRISPR-Cas12a-Based Nucleic Acid Amplification-Free DNA Biosensor via Au Nanoparticle-Assisted Metal-Enhanced Fluorescence and Colorimetric Analysis. Nano Lett. 2020; 21(1):693-699. DOI: 10.1021/acs.nanolett.0c04303. View

15.

Long Q, Tang X, Shi Q, Li Q, Deng H, Yuan J . Clinical and immunological assessment of asymptomatic SARS-CoV-2 infections. Nat Med. 2020; 26(8):1200-1204. DOI: 10.1038/s41591-020-0965-6. View

16.

Su L, Hu H, Tian Y, Jia C, Wang L, Zhang H . Highly Sensitive Colorimetric/Surface-Enhanced Raman Spectroscopy Immunoassay Relying on a Metallic Core-Shell Au/Au Nanostar with Clenbuterol as a Target Analyte. Anal Chem. 2021; 93(23):8362-8369. DOI: 10.1021/acs.analchem.1c01487. View

17.

Wang C, Yang X, Gu B, Liu H, Zhou Z, Shi L . Sensitive and Simultaneous Detection of SARS-CoV-2-Specific IgM/IgG Using Lateral Flow Immunoassay Based on Dual-Mode Quantum Dot Nanobeads. Anal Chem. 2020; 92(23):15542-15549. DOI: 10.1021/acs.analchem.0c03484. View

18.

Kohmer N, Westhaus S, Ruhl C, Ciesek S, Rabenau H . Clinical performance of different SARS-CoV-2 IgG antibody tests. J Med Virol. 2020; 92(10):2243-2247. PMC: 7300776. DOI: 10.1002/jmv.26145. View

19.

You P, Li F, Liu M, Chan Y . Colorimetric and Fluorescent Dual-Mode Immunoassay Based on Plasmon-Enhanced Fluorescence of Polymer Dots for Detection of PSA in Whole Blood. ACS Appl Mater Interfaces. 2019; 11(10):9841-9849. DOI: 10.1021/acsami.9b00204. View

20.

Camposeo A, Persano L, Manco R, Wang Y, Del Carro P, Zhang C . Metal-Enhanced Near-Infrared Fluorescence by Micropatterned Gold Nanocages. ACS Nano. 2015; 9(10):10047-54. PMC: 4625169. DOI: 10.1021/acsnano.5b03624. View