Motor Dysfunction of Gastric Antral Smooth Muscle in Diabetic Rats: Contribution of ATP-Dependent Potassium Channels

Overview

Journal Adv Biomed Res

Specialty Biomedical Engineering

Date 2023 Sep 11

PMID 37694236

Authors

Fatameh Khoshavi Najafabadi

Hassan Sadraei

Nasrin Mehranfard

Maedeh Ghasemi

Affiliations

Soon will be listed here.

Abstract

Background: The goal of the current research was to further elucidate the role of adenosine triphosphate (ATP)-sensitive potassium (K) channels in the motility and contractility force of gastric smooth muscle of diabetic rats.

Materials And Methods: Male Wistar rats (190-230 g) were grouped into control and streptozotocin (STZ)-induced diabetes (55 mg/kg) rats. Thirty days later, gastric muscle contractility was measured using a myograph and a force transducer of antral segments immersed in a tissue bath. Gastric emptying response was measured through feeding of standard pellet. Furthermore, the expression of K channel subunits in antral smooth muscle was determined by western blot technique.

Results: The amplitude of KCl-evoked twitch contractions of diabetic antral strips was about 25% more than control ( < 0.05). Application of minoxidil, a K channel opener, dose dependently decreased the force of twitch contractions in both normal and diabetic antral strips. Application of 10 μM glibenclamide, a K channel blocker, did not antagonize the minoxidil-induced relaxation of antral strips. Diabetic gastric emptying was faster than normal, although not significant. Despite the relaxant effect of minoxidil on gastric emptying rate in normal rats ( < 0.05), this effect was not observed in diabetic rats. Also, glibenclamide increased gastric emptying and antagonized minoxidil-induced relaxation in normal rats ( < 0.05). Furthermore, the expression of K Kir6.1 and SUR2B subunits was substantially reduced in antral smooth muscle in diabetic condition ( < 0.01).

Conclusion: These results propose that K channels may contribute to the development of gastric motility disorders in diabetes.

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