In Vitro Evaluation of Adhesion on Heat-Cured Resin-Based Dental Composites

Overview

Journal Materials (Basel)

Publisher MDPI

Date 2023 Sep 9

PMID 37687511

Authors

Francesco De Angelis

Simonetta DErcole

Mara Di Giulio

Mirco Vadini

Virginia Biferi

Matteo Buonvivere

Lorenzo Vanini

Luigina Cellini

Silvia Di Lodovico

Camillo DArcangelo

Affiliations

Soon will be listed here.

Abstract

Microbial adhesion on dental restorative materials may jeopardize the restorative treatment long-term outcome. The goal of this in vitro study was to assess capability to adhere and form a biofilm on the surface of heat-cured dental composites having different formulations but subjected to identical surface treatments and polymerization protocols. Three commercially available composites were evaluated: GrandioSO (GR), Venus Diamond (VD) and Enamel Plus HRi Biofunction (BF). Cylindrical specimens were prepared for quantitative determination of S5 planktonic CFU count, sessile cells CFU count and biomass optical density (OD). Qualitative Concanavalin-A assays (for extracellular polymeric substances of a biofilm matrix) and Scanning Electron Microscope (SEM) analyses (for the morphology of sessile colonies) were also performed. Focusing on planktonic CFU count, a slight but not significant reduction was observed with VD as compared to GR. Regarding sessile cells CFU count and biomass OD, a significant increase was observed for VD compared to GR and BF. Concanavalin-A assays and SEM analyses confirmed the quantitative results. Different formulations of commercially available resin composites may differently interact with . The present results showed a relatively more pronounced antiadhesive effect for BF and GR, with a reduction in sessile cells CFU count and biomass quantification.

Citing Articles

Advanced Materials for Oral Application (Volume 2).

Rusu L, Ardelean L Materials (Basel). 2025; 18(5).

PMID: 40077268 PMC: 11901160. DOI: 10.3390/ma18051042.

Influence of Polymerization Protocol on Adhesion and Proliferation of on Three Dental Composite Resins.

De Angelis F, DArcangelo C, Di Lodovico S, Sorrentino E, Buonvivere M, DErcole S Biomedicines. 2024; 12(10).

PMID: 39457548 PMC: 11505629. DOI: 10.3390/biomedicines12102235.

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