Effect of Exposure Concentration and Growth Conditions on the Association of Cerium Oxide Nanoparticles with Green Algae

Overview

Journal Nanomaterials (Basel)

Date 2023 Sep 9

PMID 37686976

Authors

Aiga Mackevica

Lyndsey Hendriks

Olga Meili-Borovinskaya

Anders Baun

Lars Michael Skjolding

Affiliations

Soon will be listed here.

Abstract

The increasing release of engineered nanoparticles (NPs) into aquatic ecosystems makes it crucial to understand the interactions of NPs with aquatic organisms, such as algae. In this study, the association of CeO NPs with unicellular algae () and changes to the cellular elemental profile were investigated using three exposure concentrations (1, 50, and 1000 µg CeO/L) at two different algal growth conditions-exponential and inhibited growth (1% glutaraldehyde). After a 24 h-exposure, algal suspensions were settled by gravity and CeO-NP/algae association was analyzed by single-cell inductively coupled plasma quadrupole mass spectrometry (sc-ICP-QMS) and ICP time-of-flight MS (sc-ICP-TOFMS). Concurrent detection of the cellular fingerprint with cerium indicated NP association with algae (adsorption/uptake) and changes in the cellular elemental profiles. Less than 5% of cells were associated with NPs when exposed to 1 µg/L. For 50 µg/L exposures in growing and inhibited cell treatments, 4% and 16% of cells were associated with CeO NPs, respectively. ICP-TOFMS analysis made it possible to exclude cellular exudates associated with CeO NPs due to the cellular fingerprint. Growing and inhibited cells had different elemental profile changes following exposure to CeO NPs-e.g., growing cells had higher Mg and lower P contents independent of CeO concentration compared to inhibited cells.

Citing Articles

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PMID: 38832127 PMC: 11145637. DOI: 10.3389/fbioe.2024.1404651.

References

Abdolahpur Monikh F, Chupani L, Arenas-Lago D, Guo Z, Zhang P, Darbha G . Particle number-based trophic transfer of gold nanomaterials in an aquatic food chain. Nat Commun. 2021; 12(1):899. PMC: 7873305. DOI: 10.1038/s41467-021-21164-w. View

Sekine R, Moore K, Matzke M, Vallotton P, Jiang H, Hughes G . Complementary Imaging of Silver Nanoparticle Interactions with Green Algae: Dark-Field Microscopy, Electron Microscopy, and Nanoscale Secondary Ion Mass Spectrometry. ACS Nano. 2017; 11(11):10894-10902. DOI: 10.1021/acsnano.7b04556. View

Bouldin J, Ingle T, Sengupta A, Alexander R, Hannigan R, Buchanan R . Aqueous toxicity and food chain transfer of Quantum DOTs in freshwater algae and Ceriodaphnia dubia. Environ Toxicol Chem. 2008; 27(9):1958-63. PMC: 3101269. DOI: 10.1897/07-637.1. View

Petersen E, Mortimer M, Burgess R, Handy R, Hanna S, Ho K . Strategies for robust and accurate experimental approaches to quantify nanomaterial bioaccumulation across a broad range of organisms. Environ Sci Nano. 2019; 6. PMC: 6774209. DOI: 10.1039/C8EN01378K. View

Booth A, Storseth T, Altin D, Fornara A, Ahniyaz A, Jungnickel H . Freshwater dispersion stability of PAA-stabilised cerium oxide nanoparticles and toxicity towards Pseudokirchneriella subcapitata. Sci Total Environ. 2014; 505:596-605. DOI: 10.1016/j.scitotenv.2014.10.010. View

Mahaye N, Musee N . Evaluation of Apical and Molecular Effects of Algae to Cerium Oxide Nanoparticles. Toxics. 2023; 11(3). PMC: 10058573. DOI: 10.3390/toxics11030283. View

Arenas-Lago D, Abdolahpur Monikh F, Vijver M, Peijnenburg W . Interaction of zero valent copper nanoparticles with algal cells under simulated natural conditions: Particle dissolution kinetics, uptake and heteroaggregation. Sci Total Environ. 2019; 689:133-140. DOI: 10.1016/j.scitotenv.2019.06.388. View

Joonas E, Aruoja V, Olli K, Kahru A . Environmental safety data on CuO and TiO nanoparticles for multiple algal species in natural water: Filling the data gaps for risk assessment. Sci Total Environ. 2018; 647:973-980. DOI: 10.1016/j.scitotenv.2018.07.446. View

Cherchi C, Chernenko T, Diem M, Gu A . Impact of nano titanium dioxide exposure on cellular structure of Anabaena variabilis and evidence of internalization. Environ Toxicol Chem. 2010; 30(4):861-9. DOI: 10.1002/etc.445. View

10.

Merrifield R, Stephan C, Lead J . Quantification of Au Nanoparticle Biouptake and Distribution to Freshwater Algae Using Single Cell - ICP-MS. Environ Sci Technol. 2018; 52(4):2271-2277. DOI: 10.1021/acs.est.7b04968. View

11.

Kosak Nee Rohder L, Brandt T, Sigg L, Behra R . Uptake and effects of cerium(III) and cerium oxide nanoparticles to Chlamydomonas reinhardtii. Aquat Toxicol. 2018; 197:41-46. DOI: 10.1016/j.aquatox.2018.02.004. View

12.

Ozkaleli M, Erdem A . Biotoxicity of TiO₂ Nanoparticles on Raphidocelis subcapitata Microalgae Exemplified by Membrane Deformation. Int J Environ Res Public Health. 2018; 15(3). PMC: 5876961. DOI: 10.3390/ijerph15030416. View

13.

Campbell C, Peden C . Chemistry. Oxygen vacancies and catalysis on ceria surfaces. Science. 2005; 309(5735):713-4. DOI: 10.1126/science.1113955. View

14.

Pace H, Rogers N, Jarolimek C, Coleman V, Higgins C, Ranville J . Determining transport efficiency for the purpose of counting and sizing nanoparticles via single particle inductively coupled plasma mass spectrometry. Anal Chem. 2011; 83(24):9361-9. PMC: 3410750. DOI: 10.1021/ac201952t. View

15.

Zheng Y, Nowack B . Meta-analysis of Bioaccumulation Data for Nondissolvable Engineered Nanomaterials in Freshwater Aquatic Organisms. Environ Toxicol Chem. 2022; 41(5):1202-1214. PMC: 9314877. DOI: 10.1002/etc.5312. View

16.

Hsiao I, Bierkandt F, Reichardt P, Luch A, Huang Y, Jakubowski N . Quantification and visualization of cellular uptake of TiO2 and Ag nanoparticles: comparison of different ICP-MS techniques. J Nanobiotechnology. 2016; 14(1):50. PMC: 4918130. DOI: 10.1186/s12951-016-0203-z. View

17.

Lekamge S, Miranda A, Ball A, Shukla R, Nugegoda D . The toxicity of coated silver nanoparticles to Daphnia carinata and trophic transfer from alga Raphidocelis subcapitata. PLoS One. 2019; 14(4):e0214398. PMC: 6447189. DOI: 10.1371/journal.pone.0214398. View

18.

Xiao R, Zheng Y . Overview of microalgal extracellular polymeric substances (EPS) and their applications. Biotechnol Adv. 2016; 34(7):1225-1244. DOI: 10.1016/j.biotechadv.2016.08.004. View

19.

Zhao J, Cao X, Liu X, Wang Z, Zhang C, White J . Interactions of CuO nanoparticles with the algae Chlorella pyrenoidosa: adhesion, uptake, and toxicity. Nanotoxicology. 2016; 10(9):1297-305. DOI: 10.1080/17435390.2016.1206149. View

20.

Yu Q, Wang Z, Zhai Y, Zhang F, Vijver M, Peijnenburg W . Effects of humic substances on the aqueous stability of cerium dioxide nanoparticles and their toxicity to aquatic organisms. Sci Total Environ. 2021; 781:146583. DOI: 10.1016/j.scitotenv.2021.146583. View