An Insight of Gene Family Through Genome-Wide Identification, Expression Profiling and Function Analysis of Under Cold Stress

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Sep 9

PMID 37686432

Authors

Hao Zhang

Yuting Ding

Kaiye Yang

Xinyu Wang

Wenshuo Gao

Qingjun Xie

Zhongyuan Liu

Caiqiu Gao

Affiliations

Soon will be listed here.

Abstract

SWEET proteins play important roles in plant growth and development, sugar loading in phloem and resistance to abiotic stress through sugar transport. In this study, 13 genes were identified from birch genome. Collinearity analysis showed that there were one tandem repeating gene pair (/) and two duplicative gene pairs (/) in the gene family. The gene promoter regions contained several cis-acting elements related to stress resistance, for example: hormone-responsive and low-temperature-responsive cis-elements. Analysis of transcriptome data showed that genes were highly expressed in several sink organs, and the most genes were rapidly up-regulated under cold stress. , which was highly expressed in cold stress, was selected for further analysis. It was found that was located on the cell membrane. After 6 h of 4 °C stress, sucrose content in the leaves and roots of transient overexpressed was significantly higher than that of the control. MDA content in roots was significantly lower than that of the control. These results indicate that may play a positive role in the response to cold stress by promoting the metabolism and transport of sucrose. In conclusion, 13 genes were identified from the whole genome level. Most of the genes of birch were expressed in the sink organs and could respond to cold stress. Transient overexpression of changed the soluble sugar content and improved the cold tolerance of birch.

Citing Articles

Expression Profiling Analysis of the SWEET Gene Family in In Vitro Pitaya Under Low-Temperature Stress and Study of Its Cold Resistance Mechanism.

Liu Y, Zhang H, Zhao K, Wei X, Li L, Tang Y Plants (Basel). 2024; 13(21).

PMID: 39520008 PMC: 11548471. DOI: 10.3390/plants13213092.

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