Anionic Phospholipids Stimulate the Proton Pumping Activity of the Plant Plasma Membrane P-Type H-ATPase

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Sep 9

PMID 37685912

Authors

Laura C Paweletz

Simon L Holtbrugge

Malina Lob

Dario De Vecchis

Lars V Schafer

Thomas Gunther Pomorski

Bo Hojen Justesen

Affiliations

Soon will be listed here.

Abstract

The activity of membrane proteins depends strongly on the surrounding lipid environment. Here, we characterize the lipid stimulation of the plant plasma membrane H-ATPase H-ATPase isoform 2 (AHA2) upon purification and reconstitution into liposomes of defined lipid compositions. We show that the proton pumping activity of AHA2 is stimulated by anionic phospholipids, especially by phosphatidylserine. This activation was independent of the cytoplasmic C-terminal regulatory domain of the pump. Molecular dynamics simulations revealed several preferential contact sites for anionic phospholipids in the transmembrane domain of AHA2. These contact sites are partially conserved in functionally different P-type ATPases from different organisms, suggesting a general regulation mechanism by the membrane lipid environment. Our findings highlight the fact that anionic lipids play an important role in the control of H-ATPase activity.

Citing Articles

Functional reconstitution of plant plasma membrane H-ATPase into giant unilamellar vesicles.

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