Methionine Addition Improves the Acid Tolerance of Lactiplantibacillus Plantarum by Altering Cellular Metabolic Flux, Energy Distribution, Lipids Composition

Overview

Journal Stress Biol

Publisher Springer Nature

Specialty Biology

Date 2023 Sep 7

PMID 37676340

Authors

Qiang Meng

Yueyao Li

Yuxin Yuan

Shaowen Wu

Kan Shi

Shuwen Liu

Affiliations

Soon will be listed here.

Abstract

This paper reported a wine-derived lactic acid bacterium, Lactiplantibacillus plantarum XJ25, which exhibited higher cell viability under acid stress upon methionine supplementation. Cellular morphology and the composition of the cytomembrane phospholipids revealed a more solid membrane architecture presented in the acid-stressed cells treated with methionine supplementation. Transcriptional analysis showed L. plantarum XJ25 reduced methionine transport and homocysteine biosynthesis under acid stress. Subsequent overexpression assays proved that methionine supplementation could alleviate the cell toxicity from homocysteine accumulation under acid stress. Finally, L. plantarum XJ25 employed energy allocation strategy to response environmental changes by balancing the uptake methionine and adjusting saturated fatty acids (SFAs) in membrane. These data support a novel mechanism of acid resistance involving methionine utilization and cellular energy distribution in LAB and provide crucial theoretical clues for the mechanisms of acid resistance in other bacteria.

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