Spectrophotometrically, Spectroscopically, Microscopically and Thermogravimetrically Optimized TiO and ZnO Nanoparticles and Their Bactericidal, Antioxidant and Cytotoxic Potential: A Novel Comparative Approach

Overview

Journal J Fluoresc

Specialties Biophysics
Chemistry

Date 2023 Sep 6

PMID 37672182

Authors

Hunaiza Tahir

Farzana Rashid

Shaukat Ali

Muhammad Summer

Rimsha Abaidullah

Affiliations

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Abstract

Current study was aimed to determine the antibacterial, antioxidant and cytotoxic potential of Titanium dioxide nanoparticles (TiONPs) and Zinc oxide nanoparticles (ZnONPs). Nanoparticles were characterized by UV-Vis spectrophotometry, particle size analyzer (PSA), fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and thermogravimetric analysis (TGA). The Minimum inhibitory concentration (MIC) was determined by standard agar dilution method. Antibacterial potential of nanoparticles was analyzed by standard disc diffusion method against bacterial strains including Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumonia. Different concentrations of NPs (0.2, 0.4, 0.6, 0.8, 1.0, 1.2 and 1.4 mg/mL) were incorporated to evaluate the antimicrobial activity. Antioxidant activity and cytotoxicity of these NPs was analyzed by DPPH method and brine shrimp cytotoxicity assay, respectively. The MIC of TiONPs against E. coli, P. aeruginosa and K. pneumoniae was 0.04, 0.08 and 0.07 mg/mL respectively while the MIC of ZnONPs against the above strains was 0.01, 0.015 and 0.01 mg/mL. The maximum zone of inhibition was observed for K. pneumoniae i.e., 20mm and 25mm against TiO and ZnO NPs respectively, at 1.4 mg/mL concentration of NPs. The susceptibility of NPs against bacterial strains was evaluated in the following order: K. pneumoniae > P. aeruginosa > E. coli. The antioxidant activity of nanoparticles increased by increasing the concentration of NPs while cytotoxic analysis exhibited non-toxic effect of ZnO NPs while TiO had toxic effects on 1.2 and 1.4 mg/mL concentrations. Results revealed that ZnO NPs have more antibacterial and negligible cytotoxic potential in contrast to TiO NPs.

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References

Chau T, Brindhadevi K, Krishnan R, Alyousef M, S Almoallim H, Whangchai N . A novel synthesis, analysis and evaluation of Musa coccinea based zero valent iron nanoparticles for antimicrobial and antioxidant. Environ Res. 2022; 209:112770. DOI: 10.1016/j.envres.2022.112770. View

Martelli G, Giacomini D . Antibacterial and antioxidant activities for natural and synthetic dual-active compounds. Eur J Med Chem. 2018; 158:91-105. DOI: 10.1016/j.ejmech.2018.09.009. View

Ghosh C, Sarkar P, Issa R, Haldar J . Alternatives to Conventional Antibiotics in the Era of Antimicrobial Resistance. Trends Microbiol. 2019; 27(4):323-338. DOI: 10.1016/j.tim.2018.12.010. View

Habib S, Rashid F, Tahir H, Liaqat I, Latif A, Naseem S . Antibacterial and Cytotoxic Effects of Biosynthesized Zinc Oxide and Titanium Dioxide Nanoparticles. Microorganisms. 2023; 11(6). PMC: 10305289. DOI: 10.3390/microorganisms11061363. View

Anupong W, On-Uma R, Jutamas K, Salmen S, Alharbi S, Joshi D . Antibacterial, antifungal, antidiabetic, and antioxidant activities potential of Coleus aromaticus synthesized titanium dioxide nanoparticles. Environ Res. 2022; 216(Pt 3):114714. DOI: 10.1016/j.envres.2022.114714. View

Joshi K, Shelar A, Kasabe U, Nikam L, Pawar R, Sangshetti J . Biofilm inhibition in Candida albicans with biogenic hierarchical zinc-oxide nanoparticles. Biomater Adv. 2022; 134:112592. DOI: 10.1016/j.msec.2021.112592. View

Iqbal J, Abbasi B, Ahmad R, Mahmood T, Ali B, Khalil A . Nanomedicines for developing cancer nanotherapeutics: from benchtop to bedside and beyond. Appl Microbiol Biotechnol. 2018; 102(22):9449-9470. DOI: 10.1007/s00253-018-9352-3. View

Naiel B, Fawzy M, Halmy M, Mahmoud A . Green synthesis of zinc oxide nanoparticles using Sea Lavender (Limonium pruinosum L. Chaz.) extract: characterization, evaluation of anti-skin cancer, antimicrobial and antioxidant potentials. Sci Rep. 2022; 12(1):20370. PMC: 9701696. DOI: 10.1038/s41598-022-24805-2. View

Sarfraz N, Khan I . Plasmonic Gold Nanoparticles (AuNPs): Properties, Synthesis and their Advanced Energy, Environmental and Biomedical Applications. Chem Asian J. 2021; 16(7):720-742. DOI: 10.1002/asia.202001202. View

10.

Zafar S, Faisal S, Jan H, Ullah R, Rizwan M, Abdullah . Development of Iron Nanoparticles (FeNPs) Using Biomass of Its Characterization, Antimicrobial, Anti-Alzheimer's, and Enzyme Inhibition Potential. Micromachines (Basel). 2022; 13(8). PMC: 9414903. DOI: 10.3390/mi13081259. View

11.

Alavi M, Nokhodchi A . Synthesis and modification of bio-derived antibacterial Ag and ZnO nanoparticles by plants, fungi, and bacteria. Drug Discov Today. 2021; 26(8):1953-1962. DOI: 10.1016/j.drudis.2021.03.030. View

12.

Salehi P, Babanouri N, Roein-Peikar M, Zare F . Long-term antimicrobial assessment of orthodontic brackets coated with nitrogen-doped titanium dioxide against Streptococcus mutans. Prog Orthod. 2018; 19(1):35. PMC: 6139290. DOI: 10.1186/s40510-018-0236-y. View

13.

Ahamed M, Khan M, Akhtar M, Alhadlaq H, Alshamsan A . Ag-doping regulates the cytotoxicity of TiO nanoparticles via oxidative stress in human cancer cells. Sci Rep. 2017; 7(1):17662. PMC: 5732217. DOI: 10.1038/s41598-017-17559-9. View

14.

Ahmad J, Siddiqui M, Akhtar M, Alhadlaq H, Alshamsan A, Khan S . Copper doping enhanced the oxidative stress-mediated cytotoxicity of TiO nanoparticles in A549 cells. Hum Exp Toxicol. 2017; 37(5):496-507. DOI: 10.1177/0960327117714040. View

15.

Imran M, Jan H, Faisal S, Shah S, Shah S, Khan M . examination of anti-parasitic, anti-Alzheimer, insecticidal and cytotoxic potential of Wallich leaves extracts. Saudi J Biol Sci. 2021; 28(5):3031-3036. PMC: 8117137. DOI: 10.1016/j.sjbs.2021.02.044. View

16.

Akhtar M, Irshad M, Ali S, Summer M, Gulrukh S, Irfan M . Evaluation of biological potential of UV-spectrophotometric, SEM, FTIR, and EDS observed Punica granatum and Plectranthus rugosus extract-coated silver nanoparticles: A comparative study. Microsc Res Tech. 2023; 87(3):616-627. DOI: 10.1002/jemt.24454. View

17.

Summer M, Tahir H, Ali S, Abaidullah R, Mumtaz S, Nawaz S . Bactericidal potential of different size sericin-capped silver nanoparticles synthesized by heat, light, and sonication. J Basic Microbiol. 2023; 63(9):1016-1029. DOI: 10.1002/jobm.202200632. View

18.

Muhammad W, Ullah N, Haroon M, Abbasi B . Optical, morphological and biological analysis of zinc oxide nanoparticles (ZnO NPs) using L. RSC Adv. 2022; 9(51):29541-29548. PMC: 9071912. DOI: 10.1039/c9ra04424h. View

19.

Baker C, Pradhan A, Pakstis L, Pochan D, Shah S . Synthesis and antibacterial properties of silver nanoparticles. J Nanosci Nanotechnol. 2005; 5(2):244-9. DOI: 10.1166/jnn.2005.034. View

20.

Tahir H, Saleem F, Ali S, Ain Q, Fazal A, Summer M . Synthesis of sericin-conjugated silver nanoparticles and their potential antimicrobial activity. J Basic Microbiol. 2020; 60(5):458-467. DOI: 10.1002/jobm.201900567. View