Genetic Variants Detected Among 106 Lymphocyte Polypeptides Observed in Two-dimensional Gels

Overview

Journal Am J Hum Genet

Publisher Cell Press

Specialty Genetics

Date 1986 Sep 1

PMID 3766538

Citations 6

Authors

S M Hanash

L J Baier

D Welch

R Kuick

M Galteau

Affiliations

Soon will be listed here.

Abstract

Peripheral blood lymphocytes from 40 children have been examined for genetic variation in their protein constituents by two-dimensional polyacrylamide gel electrophoresis. One hundred six polypeptides chosen without respect to genetic variability were scored in gels from the 40 children. For each child, gels from both parents were also examined to substantiate the genetic basis of variants observed. Of the total of 4,240 polypeptides, 23 could not be scored unambiguously. Fourteen of the polypeptides showed genetic variants in one or more of the children. One hundred twenty-nine of 4,217 polypeptides scored exhibited the combination of a normal and a variant polypeptide. All variants were present in at least one of the parents of the subject. The index of heterozygosity observed (3.05% +/- .23%) indicates substantial genetic variation in cellular protein constituents.

Citing Articles

Nonrandom distribution of structural mutants in ethylnitrosourea-treated cultured human lymphoblastoid cells.

Hanash S, Boehnke M, Chu E, Neel J, Kuick R Proc Natl Acad Sci U S A. 1988; 85(1):165-9.

PMID: 3422414 PMC: 279504. DOI: 10.1073/pnas.85.1.165.

Heterozygosity and ethnic variation in Japanese platelet proteins.

Asakawa J, Neel J, Takahashi N, SATOH C, Kaneoka S, Nishikori E Hum Genet. 1988; 78(1):1-8.

PMID: 3422209 DOI: 10.1007/BF00291224.

Estimation of mutation rates based on the analysis of polypeptide constituents of cultured human lymphoblastoid cells.

Chu E, Boehnke M, Hanash S, Kuick R, LAMB B, Neel J Genetics. 1988; 119(3):693-703.

PMID: 3402732 PMC: 1203454. DOI: 10.1093/genetics/119.3.693.

Differing amounts of genetic polymorphism in testes and male accessory glands of Drosophila melanogaster and Drosophila simulans.

Coulthart M, Singh R Biochem Genet. 1988; 26(1-2):153-64.

PMID: 3132149 DOI: 10.1007/BF00555496.

Average locus differences in mutability related to protein "class": a hypothesis.

Neel J Proc Natl Acad Sci U S A. 1990; 87(6):2062-6.

PMID: 2315302 PMC: 53626. DOI: 10.1073/pnas.87.6.2062.

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