Differential Volatile Organic Compound Expression in the Interaction of and

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2023 Sep 4

PMID 37663497

Authors

Efrain Escudero-Leyva

Luis Quiros-Guerrero

Victor Vasquez-Chaves

Reinaldo Pereira-Reyes

Priscila Chaverri

Giselle Tamayo-Castillo

Affiliations

Soon will be listed here.

Abstract

Fungi exhibit a wide range of ecological guilds, but those that live within the inner tissues of plants (also known as endophytes) are particularly relevant due to the benefits they sometimes provide to their hosts, such as herbivory deterrence, disease protection, and growth promotion. Recently, endophytes have gained interest as potential biocontrol agents against crop pathogens, for example, coffee plants (). Published results from research performed in our laboratory showed that endophytic fungi isolated from wild Rubiaceae plants were effective in reducing the effects of the American leaf spot of coffee (). One of these isolates (GU11N) from the plant was identified as (Xylariales). Its antagonism mechanisms, effects, and chemistry against were investigated by analyzing its volatile profile alone and in the presence of the pathogen in contactless and dual culture assays. The experimental design involved direct sampling of agar plugs in vials for headspace (HS) and headspace solid-phase microextraction (HS-SPME) gas chromatography-mass spectrometry (GC-MS) analysis. Additionally, we used ultrahigh-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS/MS) to identify nonvolatile compounds from organic extracts of the mycelia involved in the interaction. Results showed that more volatile compounds were identified using HS-SPME (39 components) than those by the HS technique (13 components), sharing only 12 compounds. Statistical tests suggest that inhibited the growth of through the release of VOCs containing a combination of 1,8-dimethoxynapththalene and terpene compounds affecting pseudopilei. The damaging effects of 1,8-dimethoxynaphthalene were corroborated in an in vitro test against pseudopilei; scanning electron microscopy (SEM) photographs confirmed structural damage. After analyzing the UHPLC-HRMS/MS data, a predominance of fatty acid derivatives was found among the putatively identified compounds. However, a considerable proportion of features (37.3%) remained unannotated. In conclusion, our study suggests that has potential as a biocontrol agent against and that 1,8-dimethoxynaphthalene contributes to the observed damage to the pathogen's reproductive structures.

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