Comparative Genomics Identified PenR E151V Substitution Associated with Carbapenem-Resistance Complex and a Novel Complex Specific OXA-1043 Subgroup

Overview

Journal Infect Drug Resist

Publisher Dove Medical Press

Date 2023 Sep 4

PMID 37662974

Authors

Ya-Chun Liao

Yao-Ting Huang

Chien-Hao Tseng

Chia-Wei Liu

Po-Yu Liu

Affiliations

Soon will be listed here.

Abstract

Purpose: complex (Bcc) is a known significant opportunistic pathogen causing morbidity and mortality, particularly in those with cystic fibrosis, chronic granulomatous disease, or immunocompromising host. Mortality of Bcc bloodstream infections among non-cystic fibrosis patients remained high. The antibiotic treatment for Bcc infection is quite challenging due to its intrinsic resistance to most antibiotics, and the resistance to carbapenems was the biggest concern among them. We aimed to realize the mechanism of carbapenem resistance in Bcc.

Patients And Methods: Ten strains of Bcc were identified by the MALDI-TOF MS, and the drug susceptibility test was using VITEK 2 system. The complex genomes were sequenced via Nanopore GridIon. We also downloaded another ninety-five strains of Bcc from the National Center for Biotechnology Information database to evaluate the divergence between carbapenem-resistance and carbapenem-sensitive strains.

Results: The genetic organization between carbapenem-sensitive and carbapenem-resistant strains of Bcc showed no difference. However, in the carbapenem-sensitive strain, E151V substitution in PenR was detected. In addition, a novel specific OXA family subgroup, in was discovered.

Conclusion: The E151V substitution in PenR may be associated with carbapenem-sensitive in Bcc. Moreover, the V151E mutation in PenR may be related to the activation of PenB, leading to Bcc resistance to carbapenems. Besides, a novel OXA family subgroup, , was found in , which differs from the previous OXA family.

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